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PMID: 2930493 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dendrotoxin acceptor from bovine synaptic plasma membranes. Binding properties, purification and subunit composition of a putative constituent of certain voltage-activated K+ channels.

The Biochemical journal ·Vol. 257 ·No. 3 ·1989-02-01 ·Pages 899-903

Parcej DN, Dolly JO

Abstract

Dendrotoxin is a snake polypeptide that blocks selectively and potently certain voltage-sensitive, fast-activating K+ channels in the nervous system, where it binds with high affinity to membranous acceptors. Herein, the acceptor protein for dendrotoxin in bovine synaptic membranes is solubilized in active form and its complete purification achieved by affinity chromatography, involving a novel elution procedure. This putative K+-channel constituent is shown to be a large oligomeric glycoprotein containing two major subunits, with Mr values of 75,000 and 37,000.

MeSH Terms
Animals Binding Sites Cattle Cerebral Cortex/metabolism Chromatography, Affinity Elapid Venoms/metabolism Glycoproteins/analysis,isolation & purification Neurotoxins/metabolism Oxidation-Reduction Potassium Channels/metabolism Synaptic Membranes/metabolism
Chemicals
Elapid Venoms Glycoproteins Neurotoxins Potassium Channels dendrotoxin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Parcej D N
Department of Biochemistry, Imperial College, London, U.K.
Dolly J O
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32 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2570899
Published
1989-02-01
Pages
899-903
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1135672
Subset
IM
Grants
Wellcome Trust · United Kingdom
Corrections
CommentIn
PMID: 2604736
External Links
PubMed source DOI: 10.1042/bj2570899 PMC Full Text View journal details
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