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PMID: 2922280 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Activation of interferon-regulated, dsRNA-dependent enzymes by human immunodeficiency virus-1 leader RNA.

Nucleic acids research ·Vol. 17 ·No. 3 ·1989-02-11 ·Pages 969-78

SenGupta DN, Silverman RH

Abstract

Human immunodeficiency virus-1 (HIV-1) leader RNA, which contains double-stranded regions due to inverted repeats, was shown to activate the dsRNA-dependent enzymes associated with the interferon system. HIV-1 leader RNA produced in vitro using SP6 RNA polymerase was characterized using probes for antisense and sense-strand RNA. The RNA preparation was free from significant levels of antisense RNA. HIV-1 leader RNA was shown to activate dsRNA-dependent protein kinase in a cell-free system from interferon-treated HeLa cells. Affinity resins, consisting of HIV-1 leader RNA covalently attached to cellulose, immobilized and activated dsRNA-dependent protein kinase and 2-5A-synthetase. HIV-1 leader RNA, therefore, may be a contributing factor in the mechanism by which interferon inhibits HIV replication.

MeSH Terms
2',5'-Oligoadenylate Synthetase Cell-Free System Enzyme Activation Enzymes, Immobilized HIV-1/genetics HeLa Cells Humans Interferon Type I/physiology Plasmids Protein Kinases/metabolism RNA, Double-Stranded/physiology RNA, Messenger/biosynthesis,physiology Resins, Synthetic
Chemicals
Enzymes, Immobilized Interferon Type I RNA, Double-Stranded RNA, Messenger Resins, Synthetic Protein Kinases 2',5'-Oligoadenylate Synthetase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
SenGupta D N
Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799.
Silverman R H
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.3.969
Published
1989-02-11
Pages
969-78
Language
English
Region
England
NLM ID
0411011
PMCID
PMC331716
Subset
IM
Grants
NCI NIH HHS · 1 RO1 CA 44059 · United States
External Links
PubMed source DOI: 10.1093/nar/17.3.969 PMC Full Text View journal details
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