Abstract
Both one- and two-dimensional peptide mapping techniques have been used to compare the 180-kilodalton subunits from maize chloroplast and type II nuclear DNA-dependent RNA polymerases (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6). Despite their similar molecular mass, these 180-kilodalton subunits are not the same. Also, two-dimensional tryptic maps of the 160-, 43-, and 28-kilodalton polypeptides from maize type II nuclear RNA polymerase and of the 140-, 42-, and 27-kilodalton polypeptides from maize chloroplast RNA polymerase show that each of these six polypeptides is unique.
MeSH Terms
Chloroplasts/enzymology
DNA-Directed RNA Polymerases
Macromolecular Substances
Molecular Weight
Peptide Fragments/analysis
Plants/enzymology
RNA Polymerase II
Zea mays/enzymology
Chemicals
Macromolecular Substances
Peptide Fragments
RNA Polymerase II
DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kidd G H
Bogorad L
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