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PMID: 2905037 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Impairment of yeast pre-mRNA splicing by potential secondary structure-forming sequences near the conserved branchpoint sequence.

Nucleic acids research ·Vol. 16 ·No. 22 ·1988-11-25 ·Pages 10413-23

Halfter H, Gallwitz D

Abstract

The absolutely conserved TACTAAC box within introns of RNA polymerase II-transcribed genes of the yeast Saccharomyces cerevisiae serves an indispensable role in lariat formation. We show in this report that rather short palindromic sequences inserted into the yeast actin gene intron immediately 3' to the TACTAAC box block the second but not the first splicing step. In contrast, a palindromic sequence inserted some 23 bp 3' of the TACTAAC box did not affect correct and efficient splicing. The data suggest that hairpin structures that might form adjacent to the branchsite sequence interfere with some necessary alteration of the spliceosome required for 3' intron cleavage and exon ligation.

MeSH Terms
Actins/genetics Base Sequence Exons Genes Genes, Fungal Genes, Homeobox Introns Molecular Sequence Data Nucleic Acid Conformation RNA Polymerase II/metabolism RNA Precursors/genetics RNA Splicing Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
Actins RNA Precursors RNA Polymerase II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Halfter H
Max-Planck-Institute for Biophysical Chemistry, Department of Molecular Genetics, Göttingen, FRG.
Gallwitz D
References (34)
34 references, click to expand
  1. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
  2. Isolation and sequence of the gene for actin in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3912-6 PMID: 7001447
  3. Evidence for an intron-contained sequence required for the splicing of yeast RNA polymerase II transcripts.
    Cell. 1983 Jun;33(2):519-27 PMID: 6305513
  4. Evidence for the biochemical role of an internal sequence in yeast nuclear mRNA introns: implications for U1 RNA and metazoan mRNA splicing.
    Cell. 1983 Sep;34(2):395-403 PMID: 6616616
  5. Point mutations identify the conserved, intron-contained TACTAAC box as an essential splicing signal sequence in yeast.
    Cell. 1984 Mar;36(3):645-53 PMID: 6365330
  6. Intron sequences involved in lariat formation during pre-mRNA splicing.
    Cell. 1985 May;41(1):95-105 PMID: 3888410
  7. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.
    DNA. 1985 Apr;4(2):165-70 PMID: 3996185
  8. Size and position of intervening sequences are critical for the splicing efficiency of pre-mRNA in the yeast Saccharomyces cerevisiae.
    Nucleic Acids Res. 1985 Jun 11;13(11):3791-804 PMID: 3892483
  9. An RNA processing activity that debranches RNA lariats.
    Science. 1985 Jul 12;229(4709):135-40 PMID: 2990042
  10. Molecular consequences of specific intron mutations on yeast mRNA splicing in vivo and in vitro.
    Cell. 1985 Aug;42(1):335-44 PMID: 3893746
  11. Stepwise assembly of a pre-mRNA splicing complex requires U-snRNPs and specific intron sequences.
    Cell. 1985 Aug;42(1):355-67 PMID: 3160483
  12. Cleavage of 5' splice site and lariat formation are independent of 3' splice site in yeast mRNA splicing.
    Nature. 1985 Oct 24-30;317(6039):735-7 PMID: 3903513
  13. Specific and stable intron-factor interactions are established early during in vitro pre-mRNA splicing.
    Cell. 1985 Nov;43(1):131-42 PMID: 3000599
  14. Purification of a protein required for the splicing of pre-mRNA and its separation from the lariat debranching enzyme.
    EMBO J. 1985 Dec 16;4(13A):3571-81 PMID: 4092689
  15. Mutations in a yeast intron demonstrate the importance of specific conserved nucleotides for the two stages of nuclear mRNA splicing.
    Cell. 1986 Apr 11;45(1):81-93 PMID: 3513966
  16. Yeast pre-messenger RNA splicing efficiency depends on critical spacing requirements between the branch point and 3' splice site.
    EMBO J. 1986 May;5(5):1023-30 PMID: 3013610
  17. Splicing of messenger RNA precursors.
    Annu Rev Biochem. 1986;55:1119-50 PMID: 2943217
  18. Mutations in conserved intron sequences affect multiple steps in the yeast splicing pathway, particularly assembly of the spliceosome.
    EMBO J. 1986 Jul;5(7):1683-95 PMID: 3017708
  19. U2 RNA from yeast is unexpectedly large and contains homology to vertebrate U4, U5, and U6 small nuclear RNAs.
    Cell. 1986 Oct 10;47(1):49-59 PMID: 3530502
  20. Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes.
    Nature. 1986 Nov 27-Dec 3;324(6095):341-5 PMID: 3537805
  21. Two functional alpha-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins.
    Mol Cell Biol. 1986 Nov;6(11):3711-21 PMID: 3025610
  22. Splicing of messenger RNA precursors.
    Science. 1987 Feb 13;235(4790):766-71 PMID: 3544217
  23. Pre-mRNA splicing.
    Annu Rev Genet. 1986;20:671-708 PMID: 2880558
  24. The role of small nuclear ribonucleoprotein particles in pre-mRNA splicing.
    Nature. 1987 Feb 19-25;325(6106):673-8 PMID: 2950324
  25. Effects on mRNA splicing of mutations in the 3' region of the Saccharomyces cerevisiae actin intron.
    Mol Cell Biol. 1987 Jan;7(1):225-30 PMID: 3550418
  26. Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNA.
    Cell. 1987 Apr 24;49(2):229-39 PMID: 3552247
  27. Nuclear pre-mRNA splicing in the fission yeast Schizosaccharomyces pombe strictly requires an intron-contained, conserved sequence element.
    EMBO J. 1987 Jun;6(6):1757-63 PMID: 3649292
  28. A novel role for the 3' region of introns in pre-mRNA splicing of Saccharomyces cerevisiae.
    Genes Dev. 1987 May;1(3):238-46 PMID: 3315850
  29. Splicing of yeast nuclear pre-mRNA in vitro requires a functional 40S spliceosome and several extrinsic factors.
    Genes Dev. 1987 Mar;1(1):7-18 PMID: 3322937
  30. Spliceosome assembly in yeast.
    Genes Dev. 1987 Nov;1(9):1014-27 PMID: 2962902
  31. Isolation and characterisation of a yeast chromosomal replicator.
    Nature. 1979 Nov 1;282(5734):39-43 PMID: 388229
  32. Replacement of chromosome segments with altered DNA sequences constructed in vitro.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):4951-5 PMID: 388424
  33. Structure of a split yeast gene: complete nucleotide sequence of the actin gene in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2546-50 PMID: 6994099
  34. Yeast is unable to excise foreign intervening sequences from hybrid gene transcripts.
    Proc Natl Acad Sci U S A. 1983 Mar;80(6):1496-500 PMID: 6340104
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.22.10413
Published
1988-11-25
Pages
10413-23
Language
English
Region
England
NLM ID
0411011
PMCID
PMC338914
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/16.22.10413 PMC Full Text View journal details
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