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PMID: 28986421 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Stomatal VPD Response: There Is More to the Story Than ABA.

Plant physiology ·Vol. 176 ·No. 1 ·2018-00-00 ·Pages 851-864

Merilo E, Yarmolinsky D, Jalakas P, Parik H, Tulva I, Rasulov B, Kilk K, Kollist H

Abstract

Guard cells shrink and close stomatal pores when air humidity decreases (i.e. when the difference between the vapor pressures of leaf and atmosphere [VPD] increases). The role of abscisic acid (ABA) in VPD-induced stomatal closure has been studied using ABA-related mutants that respond to VPD in some studies and not in others. The importance of ABA biosynthesis in guard cells versus vasculature for whole-plant stomatal regulation is unclear as well. Here, we show that Arabidopsis (Arabidopsis thaliana) lines carrying mutations in different steps of ABA biosynthesis as well as pea (Pisum sativum) wilty and tomato (Solanum lycopersicum) flacca ABA-deficient mutants had higher stomatal conductance compared with wild-type plants. To characterize the role of ABA production in different cells, we generated transgenic plants where ABA biosynthesis was rescued in guard cells or phloem companion cells of an ABA-deficient mutant. In both cases, the whole-plant stomatal conductance, stunted growth phenotype, and leaf ABA level were restored to wild-type values, pointing to the redundancy of ABA sources and to the effectiveness of leaf ABA transport. All ABA-deficient lines closed their stomata rapidly and extensively in response to high VPD, whereas plants with mutated protein kinase OST1 showed stunted VPD-induced responses. Another strongly ABA-insensitive mutant, defective in the six ABA PYR/RCAR receptors, responded to changes in VPD in both directions strongly and symmetrically, indicating that its VPD-induced closure could be passive hydraulic. We discuss that both the VPD-induced passive hydraulic stomatal closure and the stomatal VPD regulation of ABA-deficient mutants may be conditional on the initial pretreatment stomatal conductance.

MeSH Terms
Abscisic Acid/metabolism,pharmacology Air Arabidopsis/cytology,drug effects,genetics,physiology Biosynthetic Pathways/drug effects Green Fluorescent Proteins/metabolism Humidity Models, Biological Mutation/genetics Phenotype Phloem/cytology,drug effects Plant Stomata/cytology,drug effects,physiology Plants, Genetically Modified Signal Transduction/drug effects Vapor Pressure
Chemicals
Green Fluorescent Proteins Abscisic Acid
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Merilo Ebe ORCID
Plant Signal Research Group, Institute of Technology, University of Tartu, Tartu 50411, Estonia ebe.merilo@ut.ee.
Yarmolinsky Dmitry ORCID
Plant Signal Research Group, Institute of Technology, University of Tartu, Tartu 50411, Estonia.
Jalakas Pirko
Plant Signal Research Group, Institute of Technology, University of Tartu, Tartu 50411, Estonia.
Parik Helen ORCID
Plant Signal Research Group, Institute of Technology, University of Tartu, Tartu 50411, Estonia.
Tulva Ingmar
Plant Signal Research Group, Institute of Technology, University of Tartu, Tartu 50411, Estonia.
Rasulov Bakhtier
Plant Signal Research Group, Institute of Technology, University of Tartu, Tartu 50411, Estonia.
Kilk Kalle ORCID
Institute of Biomedicine and Translational Medicine, Faculty of Medicine, University of Tartu, Tartu 50411, Estonia.
Kollist Hannes ORCID
Plant Signal Research Group, Institute of Technology, University of Tartu, Tartu 50411, Estonia.
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
1532-2548
DOI
10.1104/pp.17.00912
Email
ebe.merilo@ut.ee
Published
2018-00-00
Epub
2017-00-06
Pages
851-864
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC5761775
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.17.00912 PMC Full Text View journal details
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