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PMID: 28868061 Published · epublish English Journal Article

Genome-Wide Identification and Expression Analysis of NRAMP Family Genes in Soybean (Glycine Max L.).

Frontiers in plant science ·Vol. 8 ·2017-00-00 ·Pages 1436

Qin L, Han P, Chen L, Walk TC, Li Y, Hu X, Xie L, Liao H, Liao X

Abstract

The NRAMP (natural resistance-associated macrophage protein) family of genes has been widely characterized in organisms ranging from bacteria to yeast, plants, mice, and humans. This gene family plays vital roles in divalent metal ion transport across cellular membranes. As yet, comprehensive analysis of NRAMP family genes has not been reported for soybean. In this study, bioinformatics analysis was conducted to identify 13 soybean NRAMP genes, along with their gene structures, phylogenetic relationships, and transmembrane domains. Expression analysis suggests that GmNRAMP genes function in numerous tissues and development stages. Moreover, soybean NRAMP genes were differentially regulated by deficiencies of N, P, K, Fe, and S, along with toxicities of Fe, Cu, Cd, and Mn. These results indicate that GmNRAMP genes function in many nutrient stress pathways, and might be involved in crosstalk among nutrient stress pathways. Subcellular localization analysis in Arabidopsis protoplasts confirmed the tonoplast or plasma membrane localization of selected soybean NRMAP proteins. Protein-protein interaction analysis found that the networks of three GmNRAMP proteins which putatively interact with nodulin-like proteins, almost distinct from the network that is common to the other 10 soybean NRAMP proteins. Subsequent qRT-PCR results confirmed that these three GmNRMAP genes exhibited enhanced expression in soybean nodules, suggesting potential functions in the transport of Fe or other metal ions in soybean nodules. Overall, the systematic analysis of the GmNRAMP gene family reported herein provides valuable information for further studies on the biological roles of GmNRAMPs in divalent metal ion transport in various soybean tissues under numerous nutrient stresses and soybean-rhizobia symbiosis.

Keywords
NRAMP gene family divalent metal toxicity nodules nutrient deficiency soybean
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Qin Lu
Key Laboratory of Biology and Genetics Improvement of Oil Crops of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural SciencesWuhan, China.
Han Peipei
Key Laboratory of Biology and Genetics Improvement of Oil Crops of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural SciencesWuhan, China.
Chen Liyu
Root Biology Center, Fujian Agriculture and Forestry UniversityFuzhou, China.
Walk Thomas C
Golden Fidelity LLCSt. Louis, MO, United States.
Li Yinshui
Key Laboratory of Biology and Genetics Improvement of Oil Crops of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural SciencesWuhan, China.
Hu Xiaojia
Key Laboratory of Biology and Genetics Improvement of Oil Crops of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural SciencesWuhan, China.
Xie Lihua
Key Laboratory of Biology and Genetics Improvement of Oil Crops of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural SciencesWuhan, China.
Liao Hong
Root Biology Center, Fujian Agriculture and Forestry UniversityFuzhou, China.
Liao Xing
Key Laboratory of Biology and Genetics Improvement of Oil Crops of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural SciencesWuhan, China.
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Article Info
Journal
Frontiers in plant science
Abbr.
Front Plant Sci
ISSN
1664-462X
DOI
10.3389/fpls.2017.01436
Published
2017-00-00
Epub
2017-00-18
Pages
1436
Language
English
Region
Switzerland
NLM ID
101568200
PMCID
PMC5563376
External Links
PubMed source DOI: 10.3389/fpls.2017.01436 PMC Full Text View journal details
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