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PMID: 23133521 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Functional characterization of 14 Pht1 family genes in yeast and their expressions in response to nutrient starvation in soybean.

PloS one ·Vol. 7 ·No. 10 ·2012-00-00 ·Pages e47726

Qin L, Guo Y, Chen L, Liang R, Gu M, Xu G, Zhao J, Walk T, Liao H

Abstract

Phosphorus (P) is essential for plant growth and development. Phosphate (Pi) transporter genes in the Pht1 family play important roles in Pi uptake and translocation in plants. Although Pht1 family genes have been well studied in model plants, little is known about their functions in soybean, an important legume crop worldwide. We identified and isolated a complete set of 14 Pi transporter genes (GmPT1-14) in the soybean genome and categorized them into two subfamilies based on phylogenetic analysis. Then, an experiment to elucidate Pi transport activity of the GmPTs was carried out using a yeast mutant defective in high-affinity Pi transport. Results showed that 12 of the 14 GmPTs were able to complement Pi uptake of the yeast mutant with Km values ranging from 25.7 to 116.3 µM, demonstrating that most of the GmPTs are high-affinity Pi transporters. Further results from qRT-PCR showed that the expressions of the 14 GmPTs differed not only in response to P availability in different tissues, but also to other nutrient stresses, including N, K and Fe deficiency, suggesting that besides functioning in Pi uptake and translocation, GmPTs might be involved in synergistic regulation of mineral nutrient homeostasis in soybean. The comprehensive analysis of Pi transporter function in yeast and expression responses to nutrition starvation of Pht1 family genes in soybean revealed their involvement in other nutrient homeostasis besides P, which could help to better understand the regulation network among ion homeostasis in plants.

MeSH Terms
Biological Transport Fungal Proteins/metabolism Gene Expression Profiling Gene Expression Regulation, Plant Genetic Complementation Test Kinetics Models, Genetic Phosphate Transport Proteins/biosynthesis,genetics Phosphates/metabolism Phylogeny Saccharomyces cerevisiae/metabolism Soybeans/metabolism
Chemicals
Fungal Proteins Phosphate Transport Proteins Phosphates
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Qin Lu
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Root Biology Center, South China Agricultural University, Guangzhou, China.
Guo Yongxiang
Chen Liyu
Liang Ruikang
Gu Mian
Xu Guohua
Zhao Jing
Walk Thomas
Liao Hong
References (56)
56 references, click to expand
  1. Phosphorus Uptake by Plants: From Soil to Cell
    Plant Physiol. 1998 Feb 1;116(2):447-53 PMID: 9490752
  2. The growing family of mitochondrial carriers in Arabidopsis.
    Trends Plant Sci. 2004 Mar;9(3):138-46 PMID: 15003237
  3. Rapid induction of regulatory and transporter genes in response to phosphorus, potassium, and iron deficiencies in tomato roots. Evidence for cross talk and root/rhizosphere-mediated signals.
    Plant Physiol. 2002 Nov;130(3):1361-70 PMID: 12428001
  4. Characterization of the rice PHO1 gene family reveals a key role for OsPHO1;2 in phosphate homeostasis and the evolution of a distinct clade in dicotyledons.
    Plant Physiol. 2010 Mar;152(3):1693-704 PMID: 20081045
  5. A Medicago truncatula phosphate transporter indispensable for the arbuscular mycorrhizal symbiosis.
    Proc Natl Acad Sci U S A. 2007 Jan 30;104(5):1720-5 PMID: 17242358
  6. The characterization of novel mycorrhiza-specific phosphate transporters from Lycopersicon esculentum and Solanum tuberosum uncovers functional redundancy in symbiotic phosphate transport in solanaceous species.
    Plant J. 2005 Apr;42(2):236-50 PMID: 15807785
  7. Molecular cloning, characterization and expression analysis of two members of the Pht1 family of phosphate transporters in Glycine max.
    PLoS One. 2011;6(6):e19752 PMID: 21698287
  8. Expression analysis suggests novel roles for members of the Pht1 family of phosphate transporters in Arabidopsis.
    Plant J. 2002 Aug;31(3):341-53 PMID: 12164813
  9. A phosphate transporter from Medicago truncatula involved in the acquisition of phosphate released by arbuscular mycorrhizal fungi.
    Plant Cell. 2002 Oct;14(10):2413-29 PMID: 12368495
  10. Symbiotic phosphate transport in arbuscular mycorrhizas.
    Trends Plant Sci. 2005 Jan;10(1):22-9 PMID: 15642520
  11. Gene expression patterns are correlated with genomic and genic structure in soybean.
    Genome. 2011 Jan;54(1):10-8 PMID: 21217801
  12. The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter.
    Mol Cell Biol. 1991 Jun;11(6):3229-38 PMID: 2038328
  13. Conservation and divergence of both phosphate- and mycorrhiza-regulated physiological responses and expression patterns of phosphate transporters in solanaceous species.
    New Phytol. 2007;173(4):817-831 PMID: 17286830
  14. The potassium transporter AtHAK5 functions in K(+) deprivation-induced high-affinity K(+) uptake and AKT1 K(+) channel contribution to K(+) uptake kinetics in Arabidopsis roots.
    Plant Physiol. 2005 Mar;137(3):1105-14 PMID: 15734909
  15. A role for auxin redistribution in the responses of the root system architecture to phosphate starvation in Arabidopsis.
    Plant Physiol. 2005 Aug;138(4):2061-74 PMID: 16040660
  16. Functional analysis and cell-specific expression of a phosphate transporter from tomato.
    Planta. 1998 Oct;206(2):225-33 PMID: 9737001
  17. Comprehensive sequence and whole-life-cycle expression profile analysis of the phosphate transporter gene family in rice.
    Mol Plant. 2011 Nov;4(6):1105-22 PMID: 21832284
  18. Changes in gene expression in Arabidopsis shoots during phosphate starvation and the potential for developing smart plants.
    Plant Physiol. 2003 Jun;132(2):578-96 PMID: 12805589
  19. Molecular mechanisms of phosphate transport in plants.
    Planta. 2002 Nov;216(1):23-37 PMID: 12430011
  20. A genome-wide transcriptional analysis using Arabidopsis thaliana Affymetrix gene chips determined plant responses to phosphate deprivation.
    Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11934-9 PMID: 16085708
  21. A novel iron-regulated metal transporter from plants identified by functional expression in yeast.
    Proc Natl Acad Sci U S A. 1996 May 28;93(11):5624-8 PMID: 8643627
  22. Characterization of two phosphate transporters from barley; evidence for diverse function and kinetic properties among members of the Pht1 family.
    Plant Mol Biol. 2003 Sep;53(1-2):27-36 PMID: 14756304
  23. RNA-Seq Atlas of Glycine max: a guide to the soybean transcriptome.
    BMC Plant Biol. 2010 Aug 05;10:160 PMID: 20687943
  24. Dynamics of periarbuscular membranes visualized with a fluorescent phosphate transporter in arbuscular mycorrhizal roots of rice.
    Plant Cell Physiol. 2010 Mar;51(3):341-53 PMID: 20097910
  25. Xenopus oocytes as an expression system for plant transporters.
    Biochim Biophys Acta. 2000 May 1;1465(1-2):343-58 PMID: 10748264
  26. Analysis of the plastidic phosphate translocator gene family in Arabidopsis and identification of new phosphate translocator-homologous transporters, classified by their putative substrate-binding site.
    Plant Physiol. 2003 Mar;131(3):1178-90 PMID: 12644669
  27. Promoter analysis of the barley Pht1;1 phosphate transporter gene identifies regions controlling root expression and responsiveness to phosphate deprivation.
    Plant Physiol. 2004 Dec;136(4):4205-14 PMID: 15542491
  28. Phosphate transporters from the higher plant Arabidopsis thaliana.
    Proc Natl Acad Sci U S A. 1996 Sep 17;93(19):10519-23 PMID: 8927627
  29. Characterization of promoter expression patterns derived from the Pht1 phosphate transporter genes of barley (Hordeum vulgare L.).
    J Exp Bot. 2004 Apr;55(398):855-65 PMID: 15020637
  30. MACRONUTRIENT UTILIZATION BY PHOTOSYNTHETIC EUKARYOTES AND THE FABRIC OF INTERACTIONS.
    Annu Rev Plant Physiol Plant Mol Biol. 2001 Jun;52:163-210 PMID: 11337396
  31. Quantitative profiling of Arabidopsis polar glycerolipids in response to phosphorus starvation. Roles of phospholipases D zeta1 and D zeta2 in phosphatidylcholine hydrolysis and digalactosyldiacylglycerol accumulation in phosphorus-starved plants.
    Plant Physiol. 2006 Oct;142(2):750-61 PMID: 16891548
  32. Rice phosphate transporters include an evolutionarily divergent gene specifically activated in arbuscular mycorrhizal symbiosis.
    Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):13324-9 PMID: 12271140
  33. Two rice phosphate transporters, OsPht1;2 and OsPht1;6, have different functions and kinetic properties in uptake and translocation.
    Plant J. 2009 Mar;57(5):798-809 PMID: 18980647
  34. Functional analysis of the Arabidopsis PHT4 family of intracellular phosphate transporters.
    New Phytol. 2008;177(4):889-898 PMID: 18086223
  35. Relationship between Energy-dependent Phosphate Uptake and the Electrical Membrane Potential in Lemna gibba G1.
    Plant Physiol. 1981 Apr;67(4):797-801 PMID: 16661757
  36. The role of nutrient availability in regulating root architecture.
    Curr Opin Plant Biol. 2003 Jun;6(3):280-7 PMID: 12753979
  37. Two cDNAs from potato are able to complement a phosphate uptake-deficient yeast mutant: identification of phosphate transporters from higher plants.
    Plant Cell. 1997 Mar;9(3):381-92 PMID: 9090882
  38. PHOSPHATE ACQUISITION.
    Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:665-693 PMID: 15012223
  39. WITHDRAWN: A phosphate transporter from the root endophytic fungus Piriformospora indica plays a role in phosphate transport to the host plant.
    J Biol Chem. 2010 Aug 20;285(34):26532-44 PMID: 20479005
  40. Mycorrhizal phosphate uptake pathway in tomato is phosphorus-repressible and transcriptionally regulated.
    New Phytol. 2009 Mar;181(4):950-959 PMID: 19140941
  41. Overlapping expression patterns and differential transcript levels of phosphate transporter genes in arbuscular mycorrhizal, Pi-fertilised and phytohormone-treated Medicago truncatula roots.
    Planta. 2009 Apr;229(5):1023-34 PMID: 19169704
  42. The phosphate transporter gene OsPht1;8 is involved in phosphate homeostasis in rice.
    Plant Physiol. 2011 Jul;156(3):1164-75 PMID: 21502185
  43. Overexpression of an Arabidopsis thaliana high-affinity phosphate transporter gene in tobacco cultured cells enhances cell growth under phosphate-limited conditions.
    Proc Natl Acad Sci U S A. 1997 Jun 24;94(13):7098-102 PMID: 9192698
  44. Closely related members of the Medicago truncatula PHT1 phosphate transporter gene family encode phosphate transporters with distinct biochemical activities.
    J Biol Chem. 2008 Sep 5;283(36):24673-81 PMID: 18596039
  45. Different pathways are involved in phosphate and iron stress-induced alterations of root epidermal cell development.
    Plant Physiol. 2001 Apr;125(4):2078-84 PMID: 11299387
  46. Differential regulation of five Pht1 phosphate transporters from maize (Zea mays L.).
    Plant Biol (Stuttg). 2006 Mar;8(2):186-97 PMID: 16547863
  47. Regulated expression of Arabidopsis phosphate transporters.
    Plant Physiol. 2002 Sep;130(1):221-33 PMID: 12226502
  48. A phosphate transporter expressed in arbuscule-containing cells in potato.
    Nature. 2001 Nov 22;414(6862):462-70 PMID: 11719809
  49. An efficient transformation procedure enabling long-term storage of competent cells of various yeast genera.
    Yeast. 1991 Oct;7(7):691-2 PMID: 1776359
  50. Knockdown of an arbuscular mycorrhiza-inducible phosphate transporter gene of Lotus japonicus suppresses mutualistic symbiosis.
    Plant Cell Physiol. 2006 Jul;47(7):807-17 PMID: 16774930
  51. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
    Nucleic Acids Res. 1994 Nov 11;22(22):4673-80 PMID: 7984417
  52. Tomato phosphate transporter genes are differentially regulated in plant tissues by phosphorus.
    Plant Physiol. 1998 Jan;116(1):91-9 PMID: 9449838
  53. An integrated transcriptome atlas of the crop model Glycine max, and its use in comparative analyses in plants.
    Plant J. 2010 Jul 1;63(1):86-99 PMID: 20408999
  54. The effect of iron on the primary root elongation of Arabidopsis during phosphate deficiency.
    Plant Physiol. 2008 Jul;147(3):1181-91 PMID: 18467463
  55. Genomic analysis of a nutrient response in Arabidopsis reveals diverse expression patterns and novel metabolic and potential regulatory genes induced by nitrate.
    Plant Cell. 2000 Aug;12(8):1491-509 PMID: 10948265
  56. Genetic regulation by NLA and microRNA827 for maintaining nitrate-dependent phosphate homeostasis in arabidopsis.
    PLoS Genet. 2011 Mar;7(3):e1002021 PMID: 21455488
Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0047726
Published
2012-00-00
Epub
2012-00-25
Pages
e47726
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC3485015
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pone.0047726 PMC Full Text View journal details
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