Home LiteratureArticle Details
PMID: 2884660 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transcriptional regulation of the tyrosine hydroxylase gene by glucocorticoid and cyclic AMP.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 11 ·1987-06-00 ·Pages 3550-4

Lewis EJ, Harrington CA, Chikaraishi DM

Abstract

Glucocorticoid and cyclic AMP increase tyrosine hydroxylase (TH) activity and mRNA levels in pheochromocytoma cultures. The transcriptional activity of the TH gene, as measured by nuclear run-on assay, is also increased when cultures are treated with the synthetic glucocorticoid dexamethasone or agents that increase intracellular cyclic AMP, such as forskolin and 8-BrcAMP. Both inducers effect transcriptional changes within 10 min after treatment and are maximal after 30 min for forskolin and after 60 min for dexamethasone. The 5' flanking sequences of the TH gene were fused to the bacterial gene chloramphenicol acetyltransferase (CAT), and the hybrid gene was transfected into pheochromocytoma cultures and GH4 pituitary cells. In both cell lines, a region of the TH gene containing bases -272 to +27 conferred induction of CAT by cyclic AMP, but not by glucocorticoid. The same results were found when a region of the TH gene containing -773 to +27 was used. Thus, the sequences required for induction of TH by cyclic AMP are contained within 272 bases of 5' flanking sequence, but sequences sufficient for glucocorticoid regulation are not contained within 773 bases.

MeSH Terms
8-Bromo Cyclic Adenosine Monophosphate/pharmacology Adrenal Gland Neoplasms Animals Base Sequence Cell Line Cloning, Molecular Colforsin/pharmacology Cyclic AMP/metabolism Dexamethasone/pharmacology Gene Expression Regulation/drug effects Genes/drug effects Genes, Regulator Kinetics Pheochromocytoma Transcription, Genetic/drug effects Tyrosine 3-Monooxygenase/genetics
Chemicals
Colforsin 8-Bromo Cyclic Adenosine Monophosphate Dexamethasone Cyclic AMP Tyrosine 3-Monooxygenase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lewis E J
Harrington C A
Chikaraishi D M
References (47)
47 references, click to expand
  1. Glucocorticoid and thyroid hormones transcriptionally regulate growth hormone gene expression.
    Proc Natl Acad Sci U S A. 1982 Dec;79(24):7659-63 PMID: 6296844
  2. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  3. Cyclic AMP regulation of lactate dehydrogenase. Isoproterenol and N6,O2-dibutyryl cyclic amp increase the rate of transcription and change the stability of lactate dehydrogenase a subunit messenger RNA in rat C6 glioma cells.
    J Biol Chem. 1983 Apr 25;258(8):5312-8 PMID: 6300127
  4. Regulation of tyrosine hydroxylase mRNA by glucocorticoid and cyclic AMP in a rat pheochromocytoma cell line. Isolation of a cDNA clone for tyrosine hydroxylase mRNA.
    J Biol Chem. 1983 Dec 10;258(23):14632-7 PMID: 6139379
  5. Glucocorticoid regulation of pro-opiomelanocortin gene transcription in the rat pituitary.
    J Biol Chem. 1984 Feb 25;259(4):2166-70 PMID: 6546571
  6. Characterization of DNA sequences through which cadmium and glucocorticoid hormones induce human metallothionein-IIA gene.
    Nature. 1984 Apr 5-11;308(5959):513-9 PMID: 6323998
  7. Detection and regulation of the tyrosine hydroxylase mRNA levels in rat adrenal medulla and brain tissues.
    Cold Spring Harb Symp Quant Biol. 1983;48 Pt 1:305-8 PMID: 6144413
  8. Nerve growth factor-mediated enzyme induction in primary cultures of bovine adrenal chromaffin cells: specificity and level of regulation.
    J Neurosci. 1984 Jul;4(7):1771-80 PMID: 6145760
  9. Alternative modes of enkephalin biosynthesis regulation by reserpine and cyclic AMP in cultured chromaffin cells.
    Proc Natl Acad Sci U S A. 1984 Jul;81(13):3949-53 PMID: 6330725
  10. Transcriptional activation of the rat liver tyrosine aminotransferase gene by cAMP.
    Proc Natl Acad Sci U S A. 1984 Nov;81(21):6637-41 PMID: 6149549
  11. Complete coding sequence of rat tyrosine hydroxylase mRNA.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):617-21 PMID: 2857492
  12. The first intron of the human growth hormone gene contains a binding site for glucocorticoid receptor.
    Proc Natl Acad Sci U S A. 1985 Feb;82(3):699-702 PMID: 2983311
  13. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.
    DNA. 1985 Apr;4(2):165-70 PMID: 3996185
  14. Cyclic AMP regulation of eukaryotic gene transcription by two discrete molecular mechanisms.
    Science. 1985 Jul 19;229(4710):267-9 PMID: 2990047
  15. Trans-synaptic increase in RNA coding for tyrosine hydroxylase in a rat sympathetic ganglion.
    Brain Res. 1985 Jul 22;339(1):151-3 PMID: 2862954
  16. Elevation of RNA coding for tyrosine hydroxylase in rat adrenal gland by reserpine treatment and exposure to cold.
    J Neurochem. 1985 Oct;45(4):1030-3 PMID: 2863328
  17. Hormonal regulation of the bovine prolactin promoter in rat pituitary tumor cells.
    J Biol Chem. 1985 Oct 5;260(22):12246-51 PMID: 2995368
  18. Corticotropin releasing factor increases proopiomelanocortin messenger RNA in mouse anterior pituitary tumor cells.
    J Biol Chem. 1985 Dec 15;260(29):15477-81 PMID: 2999118
  19. Effect of chronic cold exposure on tyrosine hydroxylase mRNA in rat adrenal gland.
    Brain Res. 1985 Dec 16;359(1-2):356-9 PMID: 2866819
  20. Cyclic AMP regulates somatostatin mRNA accumulation in primary diencephalic cultures and in transfected fibroblast cells.
    J Neurosci. 1986 Apr;6(4):1171-6 PMID: 2871140
  21. Characterization of the phosphoenolpyruvate carboxykinase (GTP) promoter-regulatory region. II. Identification of cAMP and glucocorticoid regulatory domains.
    J Biol Chem. 1986 Jul 25;261(21):9721-6 PMID: 3015903
  22. Glucocorticoid receptor binding and activation of a heterologous promoter by dexamethasone by the first intron of the human growth hormone gene.
    Mol Cell Biol. 1985 Nov;5(11):2984-92 PMID: 3018491
  23. Identification of a cyclic-AMP-responsive element within the rat somatostatin gene.
    Proc Natl Acad Sci U S A. 1986 Sep;83(18):6682-6 PMID: 2875459
  24. A cyclic AMP- and phorbol ester-inducible DNA element.
    Nature. 1986 Sep 25-Oct 1;323(6086):353-6 PMID: 3020428
  25. Induction of tyrosine hydroxylase by cyclic AMP and glucocorticoids in a rat pheochromocytoma cell line: effect of the inducing agents alone or in combination on the enzyme levels and rate of synthesis of tyrosine hydroxylase.
    Mol Pharmacol. 1986 Nov;30(5):486-96 PMID: 2430169
  26. Induction of mRNA for tyrosine hydroxylase by cyclic AMP and glucocorticoids in a rat pheochromocytoma cell line: evidence for the regulation of tyrosine hydroxylase synthesis by multiple mechanisms in cells exposed to elevated levels of both inducing agents.
    Mol Pharmacol. 1986 Nov;30(5):497-503 PMID: 2877392
  27. Identification and cell type specificity of the tyrosine hydroxylase gene promoter.
    Nucleic Acids Res. 1987 Mar 11;15(5):2363-84 PMID: 2882469
  28. Increase in tyrosine hydroxylase activity after reserpine administration.
    J Pharmacol Exp Ther. 1969 Sep;169(1):74-9 PMID: 5804613
  29. Induction of tyrosine hydroxylase in peripheral and central adrenergic neurones by cold-exposure of rats.
    Nature. 1970 Nov 28;228(5274):861-2 PMID: 5477014
  30. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  31. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.
    Proc Natl Acad Sci U S A. 1976 Jul;73(7):2424-8 PMID: 1065897
  32. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  33. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  34. Selective enzyme induction in a nerve growth factor-responsive pheochromocytoma cell line (PC 12).
    Brain Res. 1978 Oct 6;154(1):186-90 PMID: 81091
  35. Nerve growth factor-mediated induction of tyrosine hydroxylase in a clonal pheochromocytoma cell line.
    Proc Natl Acad Sci U S A. 1978 Sep;75(9):4587-90 PMID: 30087
  36. Transcriptional regulation of the ovalbumin and conalbumin genes by steroid hormones in chick oviduct.
    J Biol Chem. 1979 Sep 25;254(18):9050-8 PMID: 479179
  37. Primary cultures of chromaffin cells: molecular mechanisms for the induction of tyrosine hydroxylase mediated by 8-Br-cyclic AMP.
    Mol Pharmacol. 1979 Nov;16(3):865-76 PMID: 43468
  38. Epidermal growth factor induces tyrosine hydroxylase in a clonal pheochromocytoma cell line, PC-G2.
    J Cell Biol. 1980 Mar;84(3):495-500 PMID: 6102094
  39. Cloning DNA restriction endonuclease fragments with protruding single-stranded ends.
    Gene. 1980 May;9(3-4):307-19 PMID: 6248431
  40. Transcriptional regulation of mouse liver metallothionein-I gene by glucocorticoids.
    Nature. 1981 May 28;291(5813):340-2 PMID: 7231555
  41. Transcriptional regulation of the prolactin gene by ergocryptine and cyclic AMP.
    Nature. 1981 Nov 5;294(5836):94-7 PMID: 6270576
  42. Nerve growth factor-mediated induction of tyrosine hydroxylase and of neurite outgrowth in cultures of bovine adrenal chromaffin cells: dependence on developmental stage.
    Dev Biol. 1982 Aug;92(2):365-79 PMID: 6126414
  43. cAMP stimulates transcription of the gene for cytosolic phosphoenolpyruvate carboxykinase in rat liver nuclei.
    Proc Natl Acad Sci U S A. 1982 Sep;79(17):5137-41 PMID: 6291025
  44. Eukaryotic transcriptional regulation and chromatin-associated protein phosphorylation by cyclic AMP.
    Science. 1982 Dec 24;218(4579):1315-7 PMID: 6293056
  45. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  46. The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection.
    Proc Natl Acad Sci U S A. 1982 Nov;79(22):6777-81 PMID: 6294651
  47. Transcriptional regulation of the tryptophan oxygenase gene in rat liver by glucocorticoids.
    J Biol Chem. 1983 Apr 25;258(8):4750-3 PMID: 6187742
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.11.3550
Published
1987-06-00
Pages
3550-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304912
Subset
IM
Grants
NINDS NIH HHS · NS22675 · United States
Databases
GENBANK
M16473
External Links
PubMed source DOI: 10.1073/pnas.84.11.3550 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2