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PMID: 2876957 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Surface proteins of Bordetella pertussis: comparison of virulent and avirulent strains and effects of phenotypic modulation.

Infection and immunity ·Vol. 54 ·No. 2 ·1986-11-00 ·Pages 308-14

Armstrong SK, Parker CD

Abstract

The surface proteins of several Bordetella strains and their modulated derivatives were examined by surface radioiodination, cell fractionation, and Western blotting. A surface protein with a high Mr, missing in a mutant lacking the filamentous hemagglutinin, was identified in virulent Bordetella pertussis and Bordetella parapertussis cells and was absent in avirulent B. pertussis strains. The electrophoretic profiles of lipopolysaccharide and the 40,000-Mr anion-selective porin were not determinants which correlated with phase variation or phenotypic modulation. At least three envelope proteins (91,000, 32,000, and 30,000 molecular weight) were found only in virulent B. pertussis strains and were absent or diminished in the avirulent phase and most phenotypically modulated strains. Two transposon-induced mutants unable to produce hemolysin, dermonecrotic toxin, pertussis toxin, and filamentous hemagglutinin also lacked these three envelope proteins, confirming that virulence-associated envelope proteins were genetically regulated with other virulence-associated traits.

MeSH Terms
Antibodies, Monoclonal Bordetella pertussis/analysis,genetics,pathogenicity Cell Membrane/analysis Genetic Variation Lipopolysaccharides/analysis Membrane Proteins/analysis Molecular Weight Mutation Species Specificity Virulence
Chemicals
Antibodies, Monoclonal Lipopolysaccharides Membrane Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Armstrong S K
Parker C D
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34 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.54.2.308-314.1986
Published
1986-11-00
Pages
308-14
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260161
Subset
IM
Grants
NIAID NIH HHS · AI 17682 · United States
External Links
PubMed source DOI: 10.1128/iai.54.2.308-314.1986 PMC Full Text View journal details
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