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PMID: 6253992 Published · ppublish English Journal Article

Calmodulin activates prokaryotic adenylate cyclase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 7 ·1980-07-00 ·Pages 3841-4

Wolff J, Cook GH, Goldhammer AR, Berkowitz SA

Abstract

The adenylate cyclase of Bordetella pertussis is stimulated 100- to 1000-fold in a dose-dependent manner by calf brain calmodulin. The system has the following properties. (i) The activation is prevented by ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid and restored by Ca2+. (ii) Oxidation of the methionine residues of calmodulin abolishes the ability to activate the cyclase. (iii) Trifluoperazine inhibits calmodulin-activated cyclase. (iv) A troponin C preparation stimulates the B. pertussis cyclase with < 0.01 the potency of calmodulin. Although calmodulin has not been demonstrated in prokaryotes, this is an example of a (eukaryotic) calmodulin effect in a prokaryote.

MeSH Terms
Adenylyl Cyclases/metabolism Bordetella pertussis/enzymology Calcium/metabolism Calcium-Binding Proteins/pharmacology Calmodulin/antagonists & inhibitors,pharmacology Egtazic Acid/pharmacology Enzyme Activation/drug effects Troponin/pharmacology
Chemicals
Calcium-Binding Proteins Calmodulin Troponin Egtazic Acid Adenylyl Cyclases Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wolff J
Cook G H
Goldhammer A R
Berkowitz S A
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25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.7.3841
Published
1980-07-00
Pages
3841-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349722
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.7.3841 PMC Full Text View journal details
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