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PMID: 2874553 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Complete nucleotide and deduced amino acid sequence of bovine phenylethanolamine N-methyltransferase: partial amino acid homology with rat tyrosine hydroxylase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 15 ·1986-08-00 ·Pages 5454-8

Baetge EE, Suh YH, Joh TH

Abstract

We report here the isolation of a cDNA clone containing the full coding region of bovine phenylethanolamine N-methyltransferase (PNMTase, EC 2.1.1.28, S-adenosyl-L-methionine:phenylethanolamine N-methyltransferase). The complete nucleotide sequence of the cDNA has been determined, and the amino acid sequence of PNMTase deduced. Cultured cells transfected with an expression vector containing this cDNA produced high levels of PNMTase enzymatic activity. Antibodies specific for tyrosine hydroxylase [EC 1.14.16.2, tyrosine 3-monooxygenase; L-tyrosine, tetrahydrobiopterine: oxygen oxidoreductase (3-hydroxylating)], the first enzyme in the catecholamine pathway, possess a striking affinity for the PNMTase protein synthesized in vitro. Comparison of the deduced amino acid sequence of bovine PNMTase to rat tyrosine hydroxylase reveals that PNMTase shares significant homology with tyrosine hydroxylase and supports previous protein and immunological data suggesting that the catecholamine biosynthetic enzymes are structurally related.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Biological Evolution Cattle Cloning, Molecular Cross Reactions DNA/genetics Gene Expression Regulation Phenylethanolamine N-Methyltransferase/genetics,immunology Protein Biosynthesis Rats Transcription, Genetic Tyrosine 3-Monooxygenase/genetics,immunology
Chemicals
DNA Tyrosine 3-Monooxygenase Phenylethanolamine N-Methyltransferase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Baetge E E
Suh Y H
Joh T H
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39 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.15.5454
Published
1986-08-00
Pages
5454-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386305
Subset
IM
Grants
NIMH NIH HHS · MH24285 · United States
NINDS NIH HHS · NS19002 · United States
Databases
GENBANK
M14318
External Links
PubMed source DOI: 10.1073/pnas.83.15.5454 PMC Full Text View journal details
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