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PMID: 2852142 Published · ppublish English Journal Article

Activation of silent genes by transposons Tn5 and Tn10.

Genetics ·Vol. 120 ·No. 4 ·1988-12-00 ·Pages 875-85

Wang A, Roth JR

Abstract

The presence of transposons Tn10 or Tn5 in the genome increases the frequency with which a silent (promoter-less) gene (hisD) is mutationally activated. The activation frequency is increased 5-25-fold by Tn10 and 30-90-fold by Tn5. Activation of the hisD gene is achieved by transposition of the entire transposon or one of its flanking insertion sequences to a region just upstream of the silent gene, between a Rho-dependent termination site in the adjacent hisG gene and the hisD gene. For both Tn5 and Tn10 the component insertion sequences were found to transpose much more frequently than the entire composite element. Transposons Tn5 and Tn10 have previously been shown to carry promoters which direct transcripts into sequences adjacent to their insertion sites.

MeSH Terms
Alcohol Oxidoreductases/biosynthesis,genetics Blotting, Southern Chromosome Mapping DNA Transposable Elements Gene Expression Regulation Genes, Bacterial Mutation Promoter Regions, Genetic Salmonella typhimurium/enzymology,genetics
Chemicals
DNA Transposable Elements Alcohol Oxidoreductases histidinol dehydrogenase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wang A
Department of Biology, University of Utah, Salt Lake City 84112.
Roth J R
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/120.4.875
Published
1988-12-00
Pages
875-85
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1203580
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/120.4.875 PMC Full Text View journal details
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