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PMID: 2846743 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structure and specificity of T cell receptor gamma/delta on major histocompatibility complex antigen-specific CD3+, CD4-, CD8- T lymphocytes.

The Journal of experimental medicine ·Vol. 168 ·No. 5 ·1988-11-01 ·Pages 1899-916

Bluestone JA, Cron RQ, Cotterman M, Houlden BA, Matis LA

Abstract

Analyses of TCR-bearing murine and human T cells have defined a unique subpopulation of T cells that express the TCR-gamma/delta proteins. The specificity of TCR-gamma/delta T cells and their role in the immune response have not yet been elucidated. Here we examine alloreactive TCR-gamma/delta T cell lines and clones that recognize MHC-encoded antigens. A BALB/c nu/nu (H-2d)-derived H-2k specific T cell line and derived clones were both cytolytic and released lymphokines after recognition of a non-classical H-2 antigen encoded in the TL region of the MHC. These cells expressed the V gamma 2/C gamma 1 protein in association with a TCR-delta gene product encoded by a Va gene segment rearranged to two D delta and one J delta variable elements. A second MHC-specific B10 nu/nu (H-2b) TCR-gamma/delta T cell line appeared to recognize a classical H-2D-encoded MHC molecule and expressed a distinct V gamma/C gamma 4-encoded protein. These data suggest that many TCR-gamma/delta-expressing T cells may recognize MHC-linked antigens encoded within distinct subregions of the MHC. The role of MHC-specific TCR-gamma/delta cells in immune responses and their immunological significance are discussed.

MeSH Terms
Amino Acid Sequence Animals Antigens, Differentiation, T-Lymphocyte/physiology Base Sequence Blotting, Northern Blotting, Southern CD3 Complex Cell Line Cytotoxicity, Immunologic Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor Histocompatibility Antigens Class I/immunology Lymphocyte Activation Major Histocompatibility Complex Mice Mice, Inbred Strains Molecular Sequence Data Molecular Weight Receptors, Antigen, T-Cell/physiology,ultrastructure Receptors, Antigen, T-Cell, gamma-delta T-Lymphocytes/immunology T-Lymphocytes, Cytotoxic/immunology
Chemicals
Antigens, Differentiation, T-Lymphocyte CD3 Complex Histocompatibility Antigens Class I Receptors, Antigen, T-Cell Receptors, Antigen, T-Cell, gamma-delta
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bluestone J A
University of Chicago, Illinois 60637.
Cron R Q
Cotterman M
Houlden B A
Matis L A
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40 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.168.5.1899
Published
1988-11-01
Pages
1899-916
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2189096
Subset
IM
Grants
NCI NIH HHS · CA-14599-15 · United States
External Links
PubMed source DOI: 10.1084/jem.168.5.1899 PMC Full Text View journal details
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