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PMID: 2846585 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The M protein of vesicular stomatitis virus associates specifically with the basolateral membranes of polarized epithelial cells independently of the G protein.

The Journal of cell biology ·Vol. 107 ·No. 5 ·1988-11-00 ·Pages 1707-15

Bergmann JE, Fusco PJ

Abstract

Using monoclonal antibodies and indirect immunofluorescence microscopy, we investigated the distribution of the M protein in situ in vesicular stomatitis virus-(VSV) infected MDCK cells. M protein was observed free in the cytoplasm and associated with the plasma membrane. Using the ts045 mutant of VSV to uncouple the synthesis and transport of the VSV G protein we demonstrated that this distribution was not related to the presence of G protein on the cell surface. Sections of epon-embedded infected cells labeled with antibody to the M protein and processed for indirect horseradish peroxidase immunocytochemistry revealed that the M protein was associated specifically with the basolateral plasma membrane. The G and M proteins of VSV have therefore evolved features which bring them independently to the basolateral membrane of polarized epithelial cells and allow virus to bud specifically from that membrane.

MeSH Terms
Antibodies, Monoclonal/biosynthesis,immunology Biological Transport Cell Membrane/metabolism,microbiology Cells, Cultured Centrifugation, Density Gradient Cytoplasm/metabolism Endoplasmic Reticulum/metabolism,microbiology Enzyme-Linked Immunosorbent Assay Epithelial Cells Epithelium/microbiology Fluorescent Antibody Technique Golgi Apparatus/metabolism,microbiology Immunohistochemistry Lipid Metabolism Precipitin Tests Protein Binding Protein Processing, Post-Translational Vesicular stomatitis Indiana virus/physiology Viral Matrix Proteins/metabolism Viral Proteins/metabolism
Chemicals
Antibodies, Monoclonal Viral Matrix Proteins Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bergmann J E
Department of Anatomy and Cell Biology, Columbia University College of Physicians and Surgeons, New York 10032.
Fusco P J
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.107.5.1707
Published
1988-11-00
Pages
1707-15
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115312
Subset
IM
Grants
NIGMS NIH HHS · GM-32617 · United States
External Links
PubMed source DOI: 10.1083/jcb.107.5.1707 PMC Full Text View journal details
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