Home LiteratureArticle Details
PMID: 2846283 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nullisomic deletion of the mcf.2 transforming gene in two haemophilia B patients.

The EMBO journal ·Vol. 7 ·No. 9 ·1988-09-00 ·Pages 2795-9

Anson DS, Blake DJ, Winship PR, Birnbaum D, Brownlee GG

Abstract

The mcf.2 transforming gene sequence has been located to the region between 29 and 61 kb 3' of the factor IX gene. Two unrelated haemophilia B patients who raise antibodies to infused factor IX ('inhibitors') have deletions in excess of 273 kb encompassing the factor IX and mcf.2 genes and a CG-rich island. We believe these patients show the first nullisomic deletion of a transforming gene to be reported. No clinical condition can be attributed to the loss of the mcf.2 gene.

MeSH Terms
Blotting, Southern Chromosome Deletion Cloning, Molecular DNA/analysis,genetics DNA Probes DNA Restriction Enzymes Factor IX/genetics Hemophilia B/genetics Humans Nucleic Acid Hybridization Oncogenes Restriction Mapping
Chemicals
DNA Probes Factor IX DNA DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Anson D S
Sir William Dunn School of Pathology, University of Oxford, UK.
Blake D J
Winship P R
Birnbaum D
Brownlee G G
References (29)
29 references, click to expand
  1. A human DNA segment with properties of the gene that predisposes to retinoblastoma and osteosarcoma.
    Nature. 1986 Oct 16-22;323(6089):643-6 PMID: 2877398
  2. Loss of a c-H-ras-1 allele and aggressive human primary breast carcinomas.
    Cancer Res. 1986 Sep;46(9):4776-81 PMID: 3524819
  3. Localization of the mcf.2 transforming sequence to the X chromosome.
    EMBO J. 1987 May;6(5):1301-7 PMID: 3038515
  4. Function of a new globin gene.
    Nature. 1987 Oct 1-7;329(6138):397 PMID: 3657959
  5. Large scale physical mapping in the q27 region of the human X chromosome: the coagulation factor IX gene and the mcf.2 transforming sequence are separated by at most 270 kilobase pairs and are surrounded by several 'HTF islands'.
    EMBO J. 1987 Nov;6(11):3285-9 PMID: 2828023
  6. Randomly picked cosmid clones overlap the pyrB and oriC gap in the physical map of the E. coli chromosome.
    Nucleic Acids Res. 1988 Mar 25;16(6):2601-12 PMID: 2834694
  7. A general theory of carcinogenesis.
    Proc Natl Acad Sci U S A. 1973 Dec;70(12):3324-8 PMID: 4202843
  8. Analysis of human Y-chromosome-specific reiterated DNA in chromosome variants.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):1245-9 PMID: 265567
  9. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  10. Gene transfer by means of cell fusion I. Statistical mapping of the human X-chromosome by analysis of radiation-induced gene segregation.
    J Cell Sci. 1977 Jun;25:17-37 PMID: 561093
  11. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  12. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  13. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  14. Isolation of beta-globin-related genes from a human cosmid library.
    Gene. 1981 Apr;13(3):227-37 PMID: 6266915
  15. Rapid and efficient cosmid cloning.
    Nucleic Acids Res. 1981 Jul 10;9(13):2989-98 PMID: 6269067
  16. Analysis of transcription of the human Alu family ubiquitous repeating element by eukaryotic RNA polymerase III.
    Nucleic Acids Res. 1981 Dec 11;9(23):6439-56 PMID: 6275362
  17. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  18. Gene deletions in patients with haemophilia B and anti-factor IX antibodies.
    Nature. 1983 May 12-18;303(5913):181-2 PMID: 6843667
  19. Lambda replacement vectors carrying polylinker sequences.
    J Mol Biol. 1983 Nov 15;170(4):827-42 PMID: 6315951
  20. Carrier detection by direct gene analysis in a family with haemophilia B (factor IX deficiency).
    Lancet. 1984 Feb 4;1(8371):242-3 PMID: 6142993
  21. The gene structure of human anti-haemophilic factor IX.
    EMBO J. 1984 May;3(5):1053-60 PMID: 6329734
  22. New human transforming genes detected by a tumorigenicity assay.
    Mol Cell Biol. 1984 Sep;4(9):1695-705 PMID: 6092933
  23. Propagation of some human DNA sequences in bacteriophage lambda vectors requires mutant Escherichia coli hosts.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2880-4 PMID: 3157994
  24. Hemophilia B with inhibitor: molecular analysis of the subtotal deletion of the factor IX gene.
    Blood. 1985 Sep;66(3):728-30 PMID: 2992643
  25. Gene deletion in an Italian haemophilia B subject.
    J Med Genet. 1985 Aug;22(4):305-7 PMID: 4045960
  26. An intragenic deletion of the factor IX gene in a family with hemophilia B.
    J Clin Invest. 1985 Dec;76(6):2161-4 PMID: 3001143
  27. Alterations of myc, myb, and rasHa proto-oncogenes in cancers are frequent and show clinical correlation.
    Science. 1986 Jan 17;231(4735):261-5 PMID: 3941898
  28. CpG-rich islands and the function of DNA methylation.
    Nature. 1986 May 15-21;321(6067):209-13 PMID: 2423876
  29. Recessive cancer genes and chromosomal mechanisms in tumorigenesis.
    Ann Clin Res. 1986;18(5-6):307-13 PMID: 3032067
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-09-00
Pages
2795-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC457070
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2