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PMID: 2845237 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Protective immunity to experimental tuberculosis by mannophosphoinositides of mycobacteria.

Medical microbiology and immunology ·Vol. 177 ·No. 5 ·1988-00-00 ·Pages 265-84

Mehta PK, Khuller GK

Abstract

Mannophosphoinositides isolated from mycobacterial cells were found to induce both humoral and cell-mediated immune responses in mice when injected as mannoside-methylated bovine serum albumin (MBSA) complexes. Immunization of mice with mannoside-MBSA complexes elicited significant protection against challenge with LD50 dose of M. tuberculosis H37Rv as revealed by high survival rate, low values of root-specific lung weight, lung densities and colony forming units recovered from lung, liver and spleen, compared to the nonimmunized group. These observations were further substantiated by histopathological studies. The protective immunity elicited by mannoside-MBSA complexes against challenge with M. tuberculosis H37Rv was mediated by the cooperation of T-B cells, as shown by the passive transfer of immune cells/sera into syngeneic sublethally irradiated recipient mice.

MeSH Terms
Animals Antibodies, Bacterial/biosynthesis Cell Migration Inhibition Colony Count, Microbial Female Immunity, Cellular/drug effects Immunization, Passive Lethal Dose 50 Lung/drug effects,pathology Male Mice Mice, Inbred Strains Mycobacterium tuberculosis/analysis,growth & development,immunology Organ Size/drug effects Phosphatidylinositols/isolation & purification,therapeutic use Serum Albumin, Bovine/administration & dosage Tuberculosis, Pulmonary/immunology,mortality,physiopathology
Chemicals
Antibodies, Bacterial Phosphatidylinositols mannophosphoinositide methylated bovine serum albumin Serum Albumin, Bovine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mehta P K
Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Khuller G K
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Article Info
Journal
Medical microbiology and immunology
Abbr.
Med Microbiol Immunol
ISSN
0300-8584
DOI
10.1007/BF00189412
Published
1988-00-00
Pages
265-84
Language
English
Region
Germany
NLM ID
0314524
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00189412 View journal details
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