Home LiteratureArticle Details

PMID: 2843681 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Discontinuous transcription generates heterogeneity at the leader fusion sites of coronavirus mRNAs.

Journal of virology ·Vol. 62 ·No. 10 ·1988-10-00 ·Pages 3870-3

Makino S, Soe LH, Shieh CK, Lai MM

Abstract

Coronavirus mRNA is synthesized by a discontinuous transcription process, which involves a free leader RNA species. As a result, each virus-specific mRNA contains an identical leader RNA derived from the 5', end of the genomic RNA. In this study, we demonstrate by primer extension studies that the leader-fusion sites on a given species of coronavirus subgenomic mRNA are heterogeneous. The heterogeneity was due to variation in the number of pentanucleotide (UCUAA) repeats present at the leader fusion site. This pentanucleotide repeat region was complementary between the free leader RNA and the transcription start sites on the template RNA. This result suggests that the discontinuous transcription of coronavirus mRNAs occurs within the complementary sequences localized in two different RNA segments and that RNA joining occurs at variable sites.

MeSH Terms

Base Sequence Electrophoresis, Polyacrylamide Gel Molecular Sequence Data Murine hepatitis virus/genetics Nucleic Acid Hybridization RNA, Messenger/genetics RNA, Viral/genetics Repetitive Sequences, Nucleic Acid Transcription, Genetic

Chemicals

RNA, Messenger RNA, Viral

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Makino S

Department of Microbiology, School of Medicine, University of Southern California, Los Angeles 90033.

Soe L H

Shieh C K

Lai M M

References (25)

25 references, click to expand

Evidence for in vivo trans splicing of pre-mRNAs in tobacco chloroplasts.
Cell. 1987 Jan 16;48(1):111-9 PMID: 3791410
Evidence for trans splicing in trypanosomes.
Cell. 1986 Nov 21;47(4):527-35 PMID: 3022935
A trans-spliced leader sequence on actin mRNA in C. elegans.
Cell. 1987 Jun 19;49(6):753-61 PMID: 3581169
RNA of mouse hepatitis virus.
J Virol. 1978 May;26(2):236-42 PMID: 207885
Genomic RNA of the murine coronavirus JHM.
J Gen Virol. 1978 Nov;41(2):217-27 PMID: 214517
Sequencing end-labeled DNA with base-specific chemical cleavages.
Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
A micromethod for detailed characterization of high molecular weight RNA.
Methods Enzymol. 1980;65(1):680-7 PMID: 6154875
The replication of murine coronaviruses in enucleated cells.
Virology. 1981 Apr 15;110(1):225-30 PMID: 6259831
A unique cap(m7GpppXm)-dependent influenza virion endonuclease cleaves capped RNAs to generate the primers that initiate viral RNA transcription.
Cell. 1981 Mar;23(3):847-58 PMID: 6261960
Host cell nuclear function and murine hepatitis virus replication.
J Gen Virol. 1981 Oct;56(Pt 2):457-60 PMID: 6171615
Sequence of the nucleocapsid gene from murine coronavirus MHV-A59.
Nucleic Acids Res. 1983 Feb 11;11(3):883-91 PMID: 6687635
Presence of leader sequences in the mRNA of mouse hepatitis virus.
J Virol. 1983 Jun;46(3):1027-33 PMID: 6304334
Coronavirus JHM: nucleotide sequence of the mRNA that encodes nucleocapsid protein.
Nucleic Acids Res. 1983 Aug 11;11(15):5045-54 PMID: 6308569
Characterization of leader RNA sequences on the virion and mRNAs of mouse hepatitis virus, a cytoplasmic RNA virus.
Proc Natl Acad Sci U S A. 1984 Jun;81(12):3626-30 PMID: 6328522
Sequence and topology of a model intracellular membrane protein, E1 glycoprotein, from a coronavirus.
Nature. 1984 Apr 19-25;308(5961):751-2 PMID: 6325918
Characterization of replicative intermediate RNA of mouse hepatitis virus: presence of leader RNA sequences on nascent chains.
J Virol. 1983 Dec;48(3):633-40 PMID: 6313963
Analysis of genomic and intracellular viral RNAs of small plaque mutants of mouse hepatitis virus, JHM strain.
Virology. 1984 Nov;139(1):138-51 PMID: 6093379
Coronavirus MHV-JHM mRNA 5 has a sequence arrangement which potentially allows translation of a second, downstream open reading frame.
J Gen Virol. 1985 Mar;66 ( Pt 3):581-92 PMID: 3838338
Coding sequence of coronavirus MHV-JHM mRNA 4.
J Gen Virol. 1985 Mar;66 ( Pt 3):593-6 PMID: 3973564
Three intergenic regions of coronavirus mouse hepatitis virus strain A59 genome RNA contain a common nucleotide sequence that is homologous to the 3' end of the viral mRNA leader sequence.
J Virol. 1985 Mar;53(3):834-40 PMID: 2983094
Characterization of leader-related small RNAs in coronavirus-infected cells: further evidence for leader-primed mechanism of transcription.
Virus Res. 1985 Jul;3(1):19-33 PMID: 2992183
Leader sequences of murine coronavirus mRNAs can be freely reassorted: evidence for the role of free leader RNA in transcription.
Proc Natl Acad Sci U S A. 1986 Jun;83(12):4204-8 PMID: 3012558
Coronavirus MHV-JHM: nucleotide sequence of the mRNA that encodes the membrane protein.
Nucleic Acids Res. 1986 Aug 11;14(15):6338 PMID: 3748812
Identification of a novel Y branch structure as an intermediate in trypanosome mRNA processing: evidence for trans splicing.
Cell. 1986 Nov 21;47(4):517-25 PMID: 3779835
The 5'-end sequence of the murine coronavirus genome: implications for multiple fusion sites in leader-primed transcription.
Virology. 1987 Feb;156(2):321-30 PMID: 3027981

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.62.10.3870-3873.1988

Published

1988-10-00

Pages

3870-3

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC253535

Subset

Grants

NIAID NIH HHS · AI19244 · United States

NINDS NIH HHS · NS18146 · United States

External Links

PubMed source DOI: 10.1128/JVI.62.10.3870-3873.1988 PMC Full Text View journal details

Analysis Services

Discontinuous transcription generates heterogeneity at the leader fusion sites of coronavirus mRNAs.

Contact

WeChat Official Account