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PMID: 2843548 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sequence and developmental expression of mRNA coding for a gap junction protein in Xenopus.

The Journal of cell biology ·Vol. 107 ·No. 3 ·1988-09-00 ·Pages 1065-73

Gimlich RL, Kumar NM, Gilula NB

Abstract

Cloned complementary DNAs representing the complete coding sequence for an embryonic gap junction protein in the frog Xenopus laevis have been isolated and sequenced. The cDNAs hybridize with an RNA of 1.5 kb that is first detected in gastrulating embryos and accumulates throughout gastrulation and neurulation. By the tailbud stage, the highest abundance of the transcript is found in the region containing ventroposterior endoderm and the rudiment of the liver. In the adult, transcripts are present in the lungs, alimentary tract organs, and kidneys, but are not detected in the brain, heart, body wall and skeletal muscles, spleen, or ovary. The gene encoding this embryonic gap junction protein is present in only one or a few copies in the frog genome. In vitro translation of RNA synthesized from the cDNA template produces a 30-kD protein, as predicted by the coding sequence. This product has extensive sequence similarity to mammalian gap junction proteins in its putative transmembrane and extracellular domains, but has diverged substantially in two of its intracellular domains.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular Connexins DNA/genetics Gastrula Gene Expression Regulation Intercellular Junctions Membrane Proteins/genetics Molecular Sequence Data Nucleic Acid Hybridization Protein Biosynthesis RNA, Messenger/genetics Transcription, Genetic Xenopus laevis
Chemicals
Connexins Membrane Proteins RNA, Messenger DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gimlich R L
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Kumar N M
Gilula N B
References (55)
55 references, click to expand
  1. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  2. Metabolic coupling, ionic coupling and cell contacts.
    Nature. 1972 Feb 4;235(5336):262-5 PMID: 4551177
  3. Ribonucleic acid isolated by cesium chloride centrifugation.
    Biochemistry. 1974 Jun 4;13(12):2633-7 PMID: 4831907
  4. Low resistance junctions between mesoderm cells during development of trunk muscles.
    J Physiol. 1976 Feb;255(1):209-30 PMID: 1255515
  5. Size limit of molecules permeating the junctional membrane channels.
    Science. 1977 Jan 21;195(4275):294-6 PMID: 831276
  6. Permeability of junctions between animal cells. Intercellular transfer of nucleotides but not of macromolecules.
    Exp Cell Res. 1977 Jan;104(1):153-63 PMID: 836400
  7. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  8. A study of communication specificity between cells in culture.
    J Cell Biol. 1977 Dec;75(3):769-87 PMID: 562887
  9. Transmission of hormonal stimulation by cell-to-cell communication.
    Nature. 1978 Apr 6;272(5653):501-6 PMID: 211417
  10. Specific representation of cloned repetitive DNA sequences in sea urchin RNAs.
    Cell. 1978 Sep;15(1):189-203 PMID: 699041
  11. Permeability of the cell-to-cell membrane channels in mammalian cell juncton.
    Science. 1979 Jul 27;205(4404):404-7 PMID: 377490
  12. Communication between cells of different type.
    Nature. 1971 Jul 9;232(5306):121-2 PMID: 4933243
  13. Phosphorylation-dephosphorylation of enzymes.
    Annu Rev Biochem. 1979;48:923-59 PMID: 38740
  14. Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
    J Morphol. 1972 Feb;136(2):153-79 PMID: 4109871
  15. Homologies between gap junction proteins in lens, heart and liver.
    Nature. 1988 Feb 25;331(6158):721-3 PMID: 2830542
  16. Biogenesis of mitochondria during Xenopus laevis development.
    Dev Biol. 1972 Apr;27(4):504-18 PMID: 4337711
  17. Clonal organization of the central nervous system of the frog. I. Clones stemming from individual blastomeres of the 16-cell and earlier stages.
    Dev Biol. 1979 Aug;71(2):191-202 PMID: 499655
  18. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  19. Evidence for fixed charge in the nexus.
    Nature. 1980 May 8;285(5760):101-2 PMID: 6246436
  20. Developmental expression of two cloned sequences coding for rare sea urchin embryo messages.
    Dev Biol. 1980 May;76(2):322-40 PMID: 7190106
  21. Construction of a cloned library of expressed embryonic gene sequences from Xenopus laevis.
    Dev Biol. 1980 May;76(2):435-48 PMID: 7390012
  22. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
    Eur J Biochem. 1980 Jun;107(2):303-14 PMID: 6772444
  23. A study on the steady-state population of poly(A)+RNA during early development of Xenopus laevis.
    Dev Biol. 1980 Jun 15;77(2):431-48 PMID: 6156874
  24. Diameter of the cell-to-cell junctional membrane channels as probed with neutral molecules.
    Science. 1981 Jul 31;213(4507):551-3 PMID: 7244653
  25. Junctional intercellular communication: the cell-to-cell membrane channel.
    Physiol Rev. 1981 Oct;61(4):829-913 PMID: 6270711
  26. Comparative analysis of the major polypeptides from liver gap junctions and lens fiber junctions.
    J Cell Biol. 1982 Jan;92(1):53-9 PMID: 6173389
  27. Analysis of the sequence of amino acids surrounding sites of tyrosine phosphorylation.
    Proc Natl Acad Sci U S A. 1982 Feb;79(4):973-7 PMID: 6280176
  28. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  29. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5 PMID: 6575390
  30. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  31. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  32. Comparative analysis of the gap junction protein from rat heart and liver: is there a tissue specificity of gap junctions?
    Cell. 1983 Dec;35(2 Pt 1):539-49 PMID: 6317197
  33. Physical change in cytoplasmic messenger ribonucleoproteins in cells treated with inhibitors of mRNA transcription.
    Mol Cell Biol. 1984 Mar;4(3):415-23 PMID: 6717428
  34. Hormone-induced intercellular signal transfer dissociates cyclic AMP-dependent protein kinase.
    J Cell Biol. 1984 May;98(5):1710-9 PMID: 6327720
  35. Antibodies to gap-junctional protein selectively disrupt junctional communication in the early amphibian embryo.
    Nature. 1984 Sep 13-19;311(5982):127-31 PMID: 6088995
  36. Patterns of junctional communication in the early amphibian embryo.
    Nature. 1984 Sep 13-19;311(5982):149-51 PMID: 6206399
  37. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  38. A protein homologous to the 27,000 dalton liver gap junction protein is present in a wide variety of species and tissues.
    Cell. 1984 Nov;39(1):61-9 PMID: 6091916
  39. Cell type-specific activation of actin genes in the early amphibian embryo.
    Nature. 1984 Oct 25-31;311(5988):716-21 PMID: 6548550
  40. High-sensitivity S1 mapping with single-stranded [32P]DNA probes synthesized from bacteriophage M13mp templates.
    Gene. 1984 Oct;30(1-3):63-8 PMID: 6096224
  41. Physiology and pharmacology of gap junctions.
    Annu Rev Physiol. 1985;47:281-303 PMID: 2859833
  42. Improved fluorescent compounds for tracing cell lineage.
    Dev Biol. 1985 Jun;109(2):509-14 PMID: 2581834
  43. Single-channel currents of an intercellular junction.
    Nature. 1985 Sep 26-Oct 2;317(6035):331-5 PMID: 2413362
  44. Cell interactions and the control of gene activity during early development of Xenopus laevis.
    Dev Biol. 1986 Mar;114(1):238-46 PMID: 3956863
  45. A lens intercellular junction protein, MP26, is a phosphoprotein.
    J Cell Biol. 1986 Apr;102(4):1334-43 PMID: 3958048
  46. cAMP increases junctional conductance and stimulates phosphorylation of the 27-kDa principal gap junction polypeptide.
    Proc Natl Acad Sci U S A. 1986 Apr;83(8):2473-7 PMID: 3010311
  47. Molecular cloning of cDNA for rat liver gap junction protein.
    J Cell Biol. 1986 Jul;103(1):123-34 PMID: 3013898
  48. Cloning and characterization of human and rat liver cDNAs coding for a gap junction protein.
    J Cell Biol. 1986 Sep;103(3):767-76 PMID: 2875078
  49. Is there a common design for cell membrane channels?
    Nature. 1986 Sep 4-10;323(6083):12-3 PMID: 2427957
  50. Functional assembly of gap junction conductance in lipid bilayers: demonstration that the major 27 kd protein forms the junctional channel.
    Cell. 1987 Mar 13;48(5):733-43 PMID: 3815522
  51. Topological analysis of the major protein in isolated intact rat liver gap junctions and gap junction-derived single membrane structures.
    J Biol Chem. 1987 Jun 5;262(16):7751-63 PMID: 3034905
  52. Selective disruption of gap junctional communication interferes with a patterning process in hydra.
    Science. 1987 Jul 3;237(4810):49-55 PMID: 3037697
  53. Two homologous protein components of hepatic gap junctions.
    Nature. 1987 Oct 22-28;329(6141):732-4 PMID: 2823143
  54. Connexin43: a protein from rat heart homologous to a gap junction protein from liver.
    J Cell Biol. 1987 Dec;105(6 Pt 1):2621-9 PMID: 2826492
  55. The electrical properties of the ectoderm in the amphibian embryo during induction and early development of the nervous system.
    J Physiol. 1973 Nov;235(1):267-86 PMID: 4778140
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.107.3.1065
Published
1988-09-00
Pages
1065-73
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115294
Subset
IM
Grants
NEI NIH HHS · EY06884 · United States
NIGMS NIH HHS · GM 37904 · United States
NIGMS NIH HHS · GM11974 · United States
External Links
PubMed source DOI: 10.1083/jcb.107.3.1065 PMC Full Text View journal details
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