Home LiteratureArticle Details
PMID: 2842150 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

MSS18, a yeast nuclear gene involved in the splicing of intron aI5 beta of the mitochondrial cox1 transcript.

The EMBO journal ·Vol. 7 ·No. 5 ·1988-05-00 ·Pages 1455-64

Séraphin B, Simon M, Faye G

Abstract

We have isolated and characterized a nuclear gene, MSS18, which is implicated in the splicing of intron aI5 beta of the mitochondrial cox1 transcript (subunit 1 of the cytochrome c oxidase). Northern blotting and S1 nuclease protection experiments as well as the analysis of mitochondrial point revertants suggest that mss18 mutations block (perhaps indirectly) the cleavage of the 5' exon-intron junction of aI5 beta. Mitochondrial point revertants also indicate that up to 13 bases of the aI5 beta exon sequence, upstream of the 5' splice site of aI5 beta, are involved in vivo in the splicing of this intron. The implications of this result on the splicing of group I introns are discussed.

MeSH Terms
Amino Acid Sequence Base Sequence DNA, Fungal/genetics Electron Transport Complex IV/genetics Genes, Fungal Introns Mitochondria/metabolism Molecular Sequence Data Mutation RNA Splicing Saccharomyces cerevisiae/genetics,metabolism
Chemicals
DNA, Fungal Electron Transport Complex IV
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Séraphin B
Institut Curie-Biologie, Centre Universitaire, Orsay, France.
Simon M
Faye G
References (48)
48 references, click to expand
  1. Conservation of RNA secondary structures in two intron families including mitochondrial-, chloroplast- and nuclear-encoded members.
    EMBO J. 1983;2(1):33-8 PMID: 11894905
  2. Cloning of a nuclear gene MRS1 involved in the excision of a single group I intron (bI3) from the mitochondrial COB transcript in S. cerevisiae.
    Curr Genet. 1986;11(3):185-91 PMID: 2834089
  3. Temperature-sensitive respiratory-deficient mitochondrial mutations: isolation and genetic mapping.
    Mol Gen Genet. 1977 Apr 29;152(3):295-305 PMID: 327284
  4. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  5. Analysis of products of mitochondrial protein synthesis in yeast: genetic and biochemical aspects.
    Methods Enzymol. 1979;56:58-66 PMID: 379518
  6. Cytoduction: a tool for mitochondrial genetic studies in yeast. Utilization of the nuclear-fusion mutation kar 1-1 for transfer of drug r and mit genomes in Saccharomyces cerevisiae.
    Mol Gen Genet. 1979 Mar 5;170(3):333-44 PMID: 379549
  7. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.
    Gene. 1979 Dec;8(1):121-33 PMID: 395029
  8. The structure of transposable yeast mating type loci.
    Cell. 1980 Mar;19(3):753-64 PMID: 6244896
  9. Rapid DNA isolations for enzymatic and hybridization analysis.
    Methods Enzymol. 1980;65(1):404-11 PMID: 6246361
  10. Transcription maps of adenovirus.
    Methods Enzymol. 1980;65(1):750-68 PMID: 6246372
  11. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  12. Assembly of the mitochondrial membrane system. Structure and nucleotide sequence of the gene coding for subunit 1 of yeast cytochrme oxidase.
    J Biol Chem. 1980 Dec 25;255(24):11927-41 PMID: 6254986
  13. Sequence of introns and flanking exons in wild-type and box3 mutants of cytochrome b reveals an interlaced splicing protein coded by an intron.
    Cell. 1980 Nov;22(2 Pt 2):333-48 PMID: 7004642
  14. Codon selection in yeast.
    J Biol Chem. 1982 Mar 25;257(6):3026-31 PMID: 7037777
  15. Critical sequences within mitochondrial introns: pleiotropic mRNA maturase and cis-dominant signals of the box intron controlling reductase and oxidase.
    Cell. 1982 Apr;28(4):721-32 PMID: 6284370
  16. The making of strand-specific M13 probes.
    Gene. 1982 Mar;17(3):271-7 PMID: 7049837
  17. Internal structure of a mitochondrial intron of Aspergillus nidulans.
    Proc Natl Acad Sci U S A. 1982 Oct;79(20):6332-6 PMID: 6755468
  18. Comparison of fungal mitochondrial introns reveals extensive homologies in RNA secondary structure.
    Biochimie. 1982 Oct;64(10):867-81 PMID: 6817818
  19. Analysis of a yeast nuclear gene involved in the maturation of mitochondrial pre-messenger RNA of the cytochrome oxidase subunit I.
    Cell. 1983 Jan;32(1):77-87 PMID: 6297789
  20. A short nucleotide sequence required for regulation of HIS4 by the general control system of yeast.
    Cell. 1983 Jan;32(1):89-98 PMID: 6337724
  21. Two intron sequences in yeast mitochondrial COX1 gene: homology among URF-containing introns and strain-dependent variation in flanking exons.
    Cell. 1983 Feb;32(2):379-89 PMID: 6297792
  22. Variation, transcription and circular RNAs of the mitochondrial gene for subunit I of cytochrome c oxidase.
    J Mol Biol. 1983 Feb 15;164(1):35-58 PMID: 6188839
  23. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  24. One-step gene disruption in yeast.
    Methods Enzymol. 1983;101:202-11 PMID: 6310324
  25. Steps in processing of the mitochondrial cytochrome oxidase subunit I pre-mRNA affected by a nuclear mutation in yeast.
    Proc Natl Acad Sci U S A. 1984 Jan;81(1):8-12 PMID: 6320175
  26. Assessment of a model for intron RNA secondary structure relevant to RNA self-splicing--a review.
    Gene. 1984 Jun;28(3):277-91 PMID: 6086458
  27. Mitochondrial gene expression in saccharomyces cerevisiae. II. Fidelity of translation in isolated mitochondria from wild type and respiratory-deficient mutant cells.
    J Biol Chem. 1984 Jul 25;259(14):9332-8 PMID: 6086633
  28. The mitochondrial genome of the fission yeast Schizosaccharomyces pombe: highly homologous introns are inserted at the same position of the otherwise less conserved cox1 genes in Schizosaccharomyces pombe and Aspergillus nidulans.
    EMBO J. 1984 Sep;3(9):2129-36 PMID: 6092057
  29. A mutation in yeast mitochondrial DNA results in a precise excision of the terminal intron of the cytochrome b gene.
    J Biol Chem. 1985 Mar 25;260(6):3235-8 PMID: 3882709
  30. Self-splicing of yeast mitochondrial ribosomal and messenger RNA precursors.
    Cell. 1985 Apr;40(4):759-66 PMID: 2580635
  31. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  32. The yeast nuclear gene NAM2 is essential for mitochondrial DNA integrity and can cure a mitochondrial RNA-maturase deficiency.
    Cell. 1985 May;41(1):133-43 PMID: 2986842
  33. A mitochondrial reading frame which may code for a maturase-like protein in Saccharomyces cerevisiae.
    Nucleic Acids Res. 1985 Apr 25;13(8):3005-14 PMID: 2987871
  34. An improved DNA sequencing strategy.
    Anal Biochem. 1985 May 15;147(1):114-9 PMID: 2992313
  35. Plasmids pEMBLY: new single-stranded shuttle vectors for the recovery and analysis of yeast DNA sequences.
    Gene. 1985;35(1-2):27-32 PMID: 3896935
  36. Mitochondrial class II introns encode proteins related to the reverse transcriptases of retroviruses.
    Nature. 1985 Aug 15-21;316(6029):641-3 PMID: 2412125
  37. Characterization of a yeast nuclear gene (MST1) coding for the mitochondrial threonyl-tRNA1 synthetase.
    J Biol Chem. 1985 Dec 5;260(28):15362-70 PMID: 2999113
  38. The generality of self-splicing RNA: relationship to nuclear mRNA splicing.
    Cell. 1986 Jan 31;44(2):207-10 PMID: 2417724
  39. A self-splicing RNA excises an intron lariat.
    Cell. 1986 Jan 31;44(2):213-23 PMID: 3510741
  40. Excised group II introns in yeast mitochondria are lariats and can be formed by self-splicing in vitro.
    Cell. 1986 Jan 31;44(2):225-34 PMID: 2417726
  41. Self-splicing of group II introns in vitro: mapping of the branch point and mutational inhibition of lariat formation.
    Cell. 1986 Aug 15;46(4):557-65 PMID: 3524857
  42. Biological catalysis by RNA.
    Annu Rev Biochem. 1986;55:599-629 PMID: 2427016
  43. One binding site determines sequence specificity of Tetrahymena pre-rRNA self-splicing, trans-splicing, and RNA enzyme activity.
    Cell. 1986 Oct 24;47(2):207-16 PMID: 3021333
  44. KIN28, a yeast split gene coding for a putative protein kinase homologous to CDC28.
    EMBO J. 1986 Oct;5(10):2697-701 PMID: 3536482
  45. Interlocked RNA circle formation by a self-splicing yeast mitochondrial group I intron.
    Cell. 1987 Jan 16;48(1):101-10 PMID: 2431791
  46. A protein required for splicing group I introns in Neurospora mitochondria is mitochondrial tyrosyl-tRNA synthetase or a derivative thereof.
    Cell. 1987 Jul 31;50(3):331-45 PMID: 3607872
  47. Construction of a yeast strain devoid of mitochondrial introns and its use to screen nuclear genes involved in mitochondrial splicing.
    Proc Natl Acad Sci U S A. 1987 Oct;84(19):6810-4 PMID: 3309947
  48. A mutant of Saccharomyces cerevisiae defective for nuclear fusion.
    Proc Natl Acad Sci U S A. 1976 Oct;73(10):3651-5 PMID: 790391
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-05-00
Pages
1455-64
Language
English
Region
England
NLM ID
8208664
PMCID
PMC458396
Subset
IM
Databases
GENBANK
X07650
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2