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PMID: 2841292 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A Bacillus thuringiensis subsp. israelensis gene encoding a 125-kilodalton larvicidal polypeptide is associated with inverted repeat sequences.

Journal of bacteriology ·Vol. 170 ·No. 8 ·1988-08-00 ·Pages 3575-83

Bourgouin C, Delécluse A, Ribier J, Klier A, Rapoport G

Abstract

A gene encoding a 125-kilodalton (kDa) mosquitocidal delta-endotoxin was cloned from the 72-MDa resident plasmid of Bacillus thuringiensis subsp. israelensis. This gene is similar in its 3' region to the gene encoding the 135-kDa protein previously cloned (C. Bourgouin, A. Klier, and G. Rapoport, Mol. Gen. Genet. 205:390-397, 1986). Escherichia coli recombinant clones harboring the 125-kDa gene were toxic to larvae of the three mosquito species Aedes aegypti, Anopheles stephensi, and Culex pipiens. In addition, the B. thuringiensis subsp. israelensis DNA fragment carrying the 125-kDa protein gene contains two sets of inverted repeat sequences, identified either by the S1 nuclease method or by electron microscopic observation. The structural organization of inverted repeat sequences and of the 125-kDa gene was analyzed and suggests that this B. thuringiensis subsp. israelensis delta-endotoxin gene is located within a transposable element.

MeSH Terms
Aedes Animals Bacillus thuringiensis/genetics Bacillus thuringiensis Toxins Bacterial Proteins Bacterial Toxins Cloning, Molecular Culex DNA Restriction Enzymes DNA, Bacterial/ultrastructure Densitometry Endonucleases Endotoxins/genetics Escherichia coli/genetics Genes, Bacterial Hemolysin Proteins Immunoassay Insecticides Microscopy, Electron Nucleic Acid Hybridization Pest Control, Biological Repetitive Sequences, Nucleic Acid Single-Strand Specific DNA and RNA Endonucleases
Chemicals
Bacillus thuringiensis Toxins Bacterial Proteins Bacterial Toxins DNA, Bacterial Endotoxins Hemolysin Proteins Insecticides insecticidal crystal protein, Bacillus Thuringiensis Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bourgouin C
Département des Biotechnologies, Institut-Pasteur, Paris, France.
Delécluse A
Ribier J
Klier A
Rapoport G
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.8.3575-3583.1988
Published
1988-08-00
Pages
3575-83
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211331
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.170.8.3575-3583.1988 PMC Full Text View journal details
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