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PMID: 2836359 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and complete nucleotide sequences of the type II restriction-modification genes of Salmonella infantis.

Journal of bacteriology ·Vol. 170 ·No. 6 ·1988-06-00 ·Pages 2527-32

Karreman C, de Waard A

Abstract

The complete type II restriction-modification system of Salmonella infantis was cloned in Escherichia coli as an R . Sau3AI fragment of 3,430 base pairs. The clone was shown to express the restriction endonuclease as well as the modification methylase. The nucleotide sequence of the above fragment showed two open reading frames of 461 and 230 codons in tail-to-tail orientation. These were shown to represent the modification methylase M . SinI and the restriction endonuclease R . SinI, respectively. The methylase M . SinI amino acid sequence revealed a considerable similarity to those of other deoxycytidylate methylases. In contrast, endonuclease R . SinI did not exhibit such a similarity to other restriction enzymes.

MeSH Terms
Amino Acids/analysis Base Sequence Cloning, Molecular Codon DNA Restriction Enzymes/metabolism DNA-Cytosine Methylases Deoxyribonucleases, Type II Site-Specific Methyltransferases/metabolism Molecular Sequence Data Plasmids Salmonella/genetics Software Transcription, Genetic
Chemicals
Amino Acids Codon DNA modification methylase M.SinI DNA-Cytosine Methylases Methyltransferases DNA Restriction Enzymes Deoxyribonucleases, Type II Site-Specific GGWCC-specific type II deoxyribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Karreman C
Department of Medical Biochemistry, Sylvius Laboratories, University of Leiden, The Netherlands.
de Waard A
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.6.2527-2532.1988
Published
1988-06-00
Pages
2527-32
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211166
Subset
IM
Databases
GENBANK
J03391
External Links
PubMed source DOI: 10.1128/jb.170.6.2527-2532.1988 PMC Full Text View journal details
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