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PMID: 2836084 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structure of the cloned Locusta migratoria mitochondrial genome: restriction mapping and sequence of its ND-1 (URF-1) gene.

Current genetics ·Vol. 11 ·No. 8 ·1987-00-00 ·Pages 625-30

McCracken A, Uhlenbusch I, Gellissen G

Abstract

We have cloned the entire mitochondrial genome of Locusta migratoria in four fragments and characterised by restriction mapping. In addition, we have sequenced a 1,095 kb region containing the ND-1 (URF-1) gene. The inferred primary structure of the protein is highly homologous to its Drosophila counterpart (68%). The gene is flanked at the 5' end by the tRNA(CUNleu) gene, interrupted by the sequence TTG. The 3' end is flanked by the tRNA(UCNser) gene, followed by a sequence homologous to the 3' end of D. yakuba cytochrome b. The relative position of the genes is conserved between Locusta and Drosophila, thus indicating conservation of mitochondrial gene order in insects.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Mitochondrial/genetics Genes Grasshoppers/genetics Molecular Sequence Data Nucleotide Mapping
Chemicals
DNA, Mitochondrial DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McCracken A
Department of Biology, Queen's University, Kingston, Ontario, Canada.
Uhlenbusch I
Gellissen G
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00393926
Published
1987-00-00
Pages
625-30
Language
English
Region
United States
NLM ID
8004904
Subset
IM
Databases
GENBANK
X05286
External Links
PubMed source DOI: 10.1007/BF00393926 View journal details
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