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PMID: 2835776 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transgenic mice express the human phenylethanolamine N-methyltransferase gene in adrenal medulla and retina.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 10 ·1988-05-00 ·Pages 3648-52

Baetge EE, Behringer RR, Messing A, Brinster RL, Palmiter RD

Abstract

The human gene for phenylethanolamine N-methyltransferase (hPNMT), responsible for the conversion of norepinephrine to epinephrine, has been cloned and the complete nucleotide sequence has been determined. The structural gene consists of three exons and two introns spanning approximately equal to 2100 base pairs. Transgenic mice containing the hPNMT gene with either 2 or 8 kilobases of 5'-flanking sequence were produced and resulted in expression of hPNMT mRNA in the adrenal gland and eye. A chimeric gene consisting of 2 kilobases of the hPNMT 5'-flanking region fused to the simian virus 40 early region also resulted in tumor (T) antigen mRNA expression in adrenal glands and eyes; furthermore, immunocytochemistry showed that tumor antigen was localized in nuclei of adrenal medullary cells and cells of the inner nuclear cell layer of the retina, prominent sites of epinephrine synthesis. These results indicate that the enhancer(s) for appropriate expression of the hPNMT gene in these cell types is in the 2-kilobase 5'-flanking region of the human gene.

MeSH Terms
Adrenal Medulla/enzymology Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA Restriction Enzymes Genes Humans Mice Mice, Transgenic Molecular Sequence Data Nucleic Acid Hybridization Phenylethanolamine N-Methyltransferase/genetics Retina/enzymology
Chemicals
Phenylethanolamine N-Methyltransferase DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Baetge E E
Howard Hughes Medical Institute, University of Washington, Seattle 98195.
Behringer R R
Messing A
Brinster R L
Palmiter R D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.10.3648
Published
1988-05-00
Pages
3648-52
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC280271
Subset
IM
Grants
NCI NIH HHS · CA-38635 · United States
NICHD NIH HHS · HD-09172 · United States
NINDS NIH HHS · NS-22475 · United States
Databases
GENBANK
J03280
External Links
PubMed source DOI: 10.1073/pnas.85.10.3648 PMC Full Text View journal details
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