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PMID: 2835227 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression of the G6PD locus on the human X chromosome is associated with demethylation of three CpG islands within 100 kb of DNA.

The EMBO journal ·Vol. 7 ·No. 2 ·1988-02-00 ·Pages 401-6

Toniolo D, Martini G, Migeon BR, Dono R

Abstract

We have previously reported that expression of the G6PD locus is correlated with the methylation status of two islands of CpG dinucleotides which are 3' to the locus and in the 5' region of two adjacent genes of unknown function, P3 and GdX. We have now examined the methylation of a third CpG island in the promoter region of the G6PD gene itself in DNA from males, females and reactivants that express G6PD on the inactive X chromosome. Our results show that expression of the G6PD gene is associated with concordant demethylation of all three CpG islands in this 100-kb region of DNA.

MeSH Terms
Animals Cytidine Monophosphate/analogs & derivatives,metabolism Cytosine Nucleotides/metabolism DNA/genetics DNA Restriction Enzymes Dinucleoside Phosphates Female Genes Glucosephosphate Dehydrogenase/genetics Guanosine/analogs & derivatives,metabolism Humans Hybrid Cells/enzymology Male Methylation Mice Nucleotide Mapping Transcription, Genetic X Chromosome
Chemicals
Cytosine Nucleotides Dinucleoside Phosphates Guanosine cytidylyl-3'-5'-guanosine DNA Glucosephosphate Dehydrogenase DNA Restriction Enzymes Cytidine Monophosphate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Toniolo D
Istituto Internazionale di Genetica e Biofisica, CNR, Naples, Italy.
Martini G
Migeon B R
Dono R
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20 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-02-00
Pages
401-6
Language
English
Region
England
NLM ID
8208664
PMCID
PMC454334
Subset
IM
Grants
NICHD NIH HHS · HD 05465 · United States
External Links
PubMed source PMC Full Text View journal details
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