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PMID: 2833233 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effects of pertussis toxin on growth factor-stimulated inositol phosphate formation and DNA synthesis in Swiss 3T3 cells.

The Biochemical journal ·Vol. 249 ·No. 3 ·1988-02-01 ·Pages 917-20

Taylor CW, Blakeley DM, Corps AN, Berridge MJ, Brown KD

Abstract

We have compared the effects of pretreatment of Swiss 3T3 cell with pertussis toxin on the stimulation of DNA synthesis and phosphoinositide hydrolysis in response to a wide variety of mitogens. The toxin substantially inhibited the stimulation of DNA synthesis in response to a phorbol ester or various peptide and polypeptide growth factors irrespective of their ability to activate phosphoinositidase C. Production of inositol phosphates in response to platelet-derived growth factor, fibroblast growth factor and prostaglandin F2 alpha were unaffected by the toxin while bombesin- and vasopressin-stimulated formation of inositol phosphates were inhibited by only 27 and 23% respectively. These results argue against a major role for a pertussis toxin-sensitive G protein in coupling any of these mitogen receptors to activation of a phosphoinositidase C. Furthermore, the results suggest that the widespread inhibitory effects of pertussis toxin on mitogen-stimulated DNA synthesis may be unrelated to the toxin's limited actions on phosphoinositide hydrolysis.

MeSH Terms
Animals Bombesin/pharmacology Cell Line DNA/biosynthesis Growth Substances/pharmacology Inositol Phosphates/metabolism Mice Pertussis Toxin Phosphatidylinositols/metabolism Sugar Phosphates/metabolism Thymidine/metabolism Virulence Factors, Bordetella/pharmacology
Chemicals
Growth Substances Inositol Phosphates Phosphatidylinositols Sugar Phosphates Virulence Factors, Bordetella DNA Pertussis Toxin Bombesin Thymidine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Taylor C W
AFRC Unit of Insect Neurophysiology and Pharmacology, Department of Zoology, Cambridge, U.K.
Blakeley D M
Corps A N
Berridge M J
Brown K D
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2490917
Published
1988-02-01
Pages
917-20
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1148796
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2490917 PMC Full Text View journal details
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