Intergeneric and intrageneric conjugal transfer of plasmid-encoded antibiotic resistance determinants in Leuconostoc spp.

Applied and environmental microbiology ·Vol. 54 ·No. 2 ·1988-02-00 ·Pages 281-7

Pucci MJ, Monteschio ME, Kemker CL

Abstract

Transfer of the broad-host-range resistance plasmids pIP501 and pAM beta 1 from Streptococcus faecalis to Leuconostoc dextranicum and Leuconostoc cremoris occurred between cells that were immobilized on nitrocellulose filters in the presence of DNase. Transfer of pIP501 to Leuconostoc spp. also occurred when Streptococcus sanguis and Streptococcus lactis were used as donors. In addition, transfer of pIP501 and pAM beta 1 was observed from L. cremoris and L. dextranicum transconjugants to S. sanguis and S. faecalis. Expression of the pAM beta 1 erythromycin and pIP501 erythromycin and chloramphenicol resistance determinants was essentially equivalent in donors and transconjugants. Frequencies of transfer generally ranged from 10(-4) to 10(-7) transconjugants per input donor cell. Intrageneric transfer of pIP501 and pAM beta 1 occurred between L. cremoris and L. dextranicum strains in the same approximate range. These data further extend the host range of pIP501 and pAM beta 1 and demonstrate another example of gene transfer in the genus Leuconostoc.

MeSH Terms

Chloramphenicol/pharmacology Chloramphenicol Resistance/genetics Conjugation, Genetic DNA Restriction Enzymes DNA, Bacterial/analysis Deoxyribonucleases Deoxyribonucleases, Type II Site-Specific Drug Resistance, Microbial/genetics Electrophoresis, Agar Gel Enterococcus faecalis/drug effects,genetics Erythromycin/pharmacology Genes, Bacterial Lactococcus lactis/drug effects,genetics Leuconostoc/drug effects,genetics R Factors Streptococcus sanguis/drug effects,genetics Transfection

Chemicals

DNA, Bacterial Erythromycin Chloramphenicol Deoxyribonucleases DNA Restriction Enzymes endodeoxyribonuclease PaeI CAGCTG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Pucci M J

Microlife Technics, Sarasota, Florida 34230.

Monteschio M E

Kemker C L

References (16)

16 references, click to expand

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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.54.2.281-287.1988

Published

1988-02-00

Pages

281-7

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC202444

Subset

External Links

PubMed source DOI: 10.1128/aem.54.2.281-287.1988 PMC Full Text View journal details

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Intergeneric and intrageneric conjugal transfer of plasmid-encoded antibiotic resistance determinants in Leuconostoc spp.

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