"Conjugal" transfer of plasmid DNA among oral streptococci.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 7 ·1978-07-00 ·Pages 3484-7

LeBlanc DJ, Hawley RJ, Lee LN, St Martin EJ

Abstract

The beta plasmid from Streptococcus faecalis strain DS5, which codes for resistance to erythromycin and lincomycin, was introduced into a Lancefield group F streptococcus, strain DR1501, by transformation. This strain, DR1501 (beta), was found to be an excellent donor of the beta plasmid and readily transferred the resistance markers to various lactic acid bacteria, including certain strains of S. mutans, S. sanguis, and S. salivarius. Evidence is presented indicating that the transfer of the beta plasmid is mediated by a mechanism that requires cell-to-cell contact. The transfer of plasmid DNA during conjugation has been confirmed by the isolation of beta plasmid from transconjugant clones and by their ability to then serve as donors of the erythromycin and lincomycin resistance markers.

MeSH Terms

Conjugation, Genetic/drug effects Deoxyribonucleases/metabolism Erythromycin/pharmacology Lincomycin/pharmacology Magnesium/pharmacology Plasmids R Factors Streptococcus/genetics Streptococcus mutans/genetics Transformation, Bacterial

Chemicals

Erythromycin Lincomycin Deoxyribonucleases Magnesium

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

LeBlanc D J

Hawley R J

Lee L N

St Martin E J

References (13)

13 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.75.7.3484

Published

1978-07-00

Pages

3484-7

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC392802

Subset

External Links

PubMed source DOI: 10.1073/pnas.75.7.3484 PMC Full Text View journal details

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"Conjugal" transfer of plasmid DNA among oral streptococci.

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