Home LiteratureArticle Details

PMID: 2832393 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transposition of IS50L activates downstream genes.

Journal of bacteriology ·Vol. 170 ·No. 4 ·1988-04-00 ·Pages 1965-8

Kendrick KE, Reznikoff WS

Abstract

A transposition system constructed to detect the transposition of Tn5 to a site upstream of the lacZ gene has revealed that transposition of IS50L can activate downstream genes. Expression is apparently mediated by the NPTII promoter. Transposase produced either by IS50R or by the suppressed IS50L catalyzed transposition of IS50L.

MeSH Terms

DNA Restriction Enzymes DNA Transposable Elements Drug Resistance, Microbial/genetics Electrophoresis, Agar Gel Escherichia coli/genetics Gene Expression Regulation Genes, Bacterial Mutation Plasmids Promoter Regions, Genetic

Chemicals

DNA Transposable Elements DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kendrick K E

Department of Biochemistry, University of Wisconsin, Madison 53706.

Reznikoff W S

References (16)

16 references, click to expand

Transposition of R factor genes to bacteriophage lambda.
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3628-32 PMID: 1059152
Lactose promoter mutation Pr115 activates an overlapping promoter within the lactose control region.
J Mol Biol. 1985 Oct 5;185(3):525-33 PMID: 2414453
DNA sequencing with chain-terminating inhibitors.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance.
Mol Gen Genet. 1979;177(1):65-72 PMID: 231729
The inverted repeats of Tn5 are functionally different.
Cell. 1980 Mar;19(3):795-805 PMID: 6244898
Polarity of Tn5 insertion mutations in Escherichia coli.
J Bacteriol. 1980 May;142(2):439-46 PMID: 6247321
The functional differences in the inverted repeats of Tn5 are caused by a single base pair nonhomology.
Cell. 1981 Jan;23(1):191-9 PMID: 6260374
Structural analysis of Tn5.
Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:107-13 PMID: 6271452
Insertion, excision, and inversion of Tn5.
Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:115-23 PMID: 6271454
Replicon fusions promoted by the inverted repeats of Tn5. The right repeat is an insertion sequence.
J Mol Biol. 1981 Jul 25;150(1):15-32 PMID: 6271973
Structure and stability of transposon 5-mediated cointegrates.
J Mol Biol. 1982 Aug 25;159(4):557-80 PMID: 6292435
Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5.
Gene. 1982 Oct;19(3):327-36 PMID: 6295884
Copy number control of Tn5 transposition.
Genetics. 1984 May;107(1):9-18 PMID: 6327462
Role of the IS50 R proteins in the promotion and control of Tn5 transposition.
J Mol Biol. 1984 Aug 25;177(4):645-61 PMID: 6090674
Completion of the nucleotide sequence of the central region of Tn5 confirms the presence of three resistance genes.
Nucleic Acids Res. 1985 Jan 11;13(1):195-205 PMID: 3889831
Tryptophan-transducing bacteriophages: in vitro studies with restriction endonucleases HindII + III and Escherichia coli ribonucleic acid polymerase.
J Bacteriol. 1977 Oct;132(1):270-81 PMID: 334724

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.170.4.1965-1968.1988

Published

1988-04-00

Pages

1965-8

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC211060

Subset

Grants

NCI NIH HHS · CA09075 · United States

NIGMS NIH HHS · GM19670 · United States

External Links

PubMed source DOI: 10.1128/jb.170.4.1965-1968.1988 PMC Full Text View journal details

Analysis Services

Transposition of IS50L activates downstream genes.

Contact

WeChat Official Account