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PMID: 2832376 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

narI region of the Escherichia coli nitrate reductase (nar) operon contains two genes.

Journal of bacteriology ·Vol. 170 ·No. 4 ·1988-04-00 ·Pages 1721-9

Sodergren EJ, DeMoss JA

Abstract

In previous studies it has been established that in Escherichia coli the three known subunits of anaerobic nitrate reductase are encoded by the narGHI operon. From the nucleotide sequence of the narI region of the operon we conclude that, in addition to the narG and narH genes, the nar operon contains two other open reading frames (ORFs), ORF1 and ORF2, that encode proteins of 26.5 and 25.5 kilodaltons, respectively. Protein fusions to each of the genes in the operon showed that expression of all four genes was similarly regulated. The reading frames of ORF1 and ORF2 were verified, and the N-terminal sequence for the ORF1 fusion protein was determined. The nar operon therefore contains four genes designated and ordered as narGHJI.

MeSH Terms
Anaerobiosis Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Bacterial/genetics Escherichia coli/enzymology,genetics Gene Expression Regulation Genes, Bacterial Molecular Sequence Data Nitrate Reductase Nitrate Reductases/genetics Nitrates/metabolism Operon Plasmids Repetitive Sequences, Nucleic Acid Transformation, Bacterial beta-Galactosidase/genetics
Chemicals
DNA, Bacterial Nitrates Nitrate Reductases Nitrate Reductase DNA Restriction Enzymes beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sodergren E J
Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston 77025.
DeMoss J A
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.4.1721-1729.1988
Published
1988-04-00
Pages
1721-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211023
Subset
IM
Grants
NIGMS NIH HHS · GM19511 · United States
Databases
GENBANK
M20147
External Links
PubMed source DOI: 10.1128/jb.170.4.1721-1729.1988 PMC Full Text View journal details
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