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PMID: 28305777 Published · ppublish English Journal Article

A mesoderm-inducing factor from a Xenopus laevis cell line : Chemical properties and relation to the vegetalizing factor from chicken embryos.

Roux's archives of developmental biology : the official organ of the EDBO ·Vol. 198 ·No. 1 ·1989-05-00 ·Pages 8-13

Grunz H, Born J, Davids M, Hoppe P, Loppnow-Blinde B, Tacke L, Tiedemann H, Tiedemann H

Abstract

We have compared the chemical properties and biological activities of the mesoderm-inducing factor that is secreted by the Xenopus XTC cell line with the vegetalizing factor from chicken embryos. The inducing activity of the factors was tested in different concentrations on totipotent ectoderm either by implantation into early gastrulae of Triturm alpestris or by application of solutions to isolated ectoderm of early gastrulae of Xenopus laevis. Both factors have similar properties. They are not irreversibly inactivated after treatment with 6 M urea or with phenol at 60° C. Reduction with thioglycolic acid inactivates the factors completely. The inducing activity of XTC-conditioned medium decreases only slightly after treatment with 50% formic acid. The apparent molecular mass and the isoelectric point of the factors are similar. The XTC factor was partially purified by size-exclusion and reversed-phase high-pressure liquid chromatography and by isoelectric focusing. The possible relationship of these factors to transforming growth factor β is discussed.

Keywords
Chicken vegetalizing factor Mesoderm induction Xenopus XTC factor
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Grunz Horst
Abteilung für Zoophysiologie Universität-GHS-Essen, Universitätsstraße 5, D-4300, Essen 1, Germany.
Born Jochen
Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-1000, Berlin 33.
Davids Michael
Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-1000, Berlin 33.
Hoppe Peter
Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-1000, Berlin 33.
Loppnow-Blinde Beate
Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-1000, Berlin 33.
Tacke Lothar
Abteilung für Zoophysiologie Universität-GHS-Essen, Universitätsstraße 5, D-4300, Essen 1, Germany.
Tiedemann Heinz
Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-1000, Berlin 33.
Tiedemann Hildegard
Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-1000, Berlin 33.
References (34)
34 references, click to expand
  1. Mesoderm-inducing factors: a small class of molecules.
    Development. 1988 Mar;102(3):555-66 PMID: 3181034
  2. Purification, partial characterization and biological effects of the XTC mesoderm-inducing factor.
    Development. 1988 Jul;103(3):591-600 PMID: 3246228
  3. A mesoderm-inducing factor is produced by Xenopus cell line.
    Development. 1987 Jan;99(1):3-14 PMID: 15005106
  4. Inducing activity of subcellular fractions from amphibian embryos.
    Wilehm Roux Arch Dev Biol. 1984 Jan;193(1):1-12 PMID: 28305490
  5. Cleavage at aspartyl-prolyl bonds.
    Methods Enzymol. 1977;47:145-9 PMID: 22018
  6. A vegetalizing inducing factor. Isolation and chemical properties.
    Biochim Biophys Acta. 1981 Sep 4;676(3):350-6 PMID: 7284443
  7. Inducing effects of the presumptive endoderm of successive stages inTriturus alpestris.
    Wilehm Roux Arch Dev Biol. 1975 Dec;177(4):301-308 PMID: 28304998
  8. Mesoderm induction in amphibians: the role of TGF-beta 2-like factors.
    Science. 1988 Feb 12;239(4841 Pt 1):783-5 PMID: 3422517
  9. Isolation of a vegetalizing inducing factor.
    Hoppe Seylers Z Physiol Chem. 1972 Jul;353(7):1075-84 PMID: 5073869
  10. Affinity chromatography of embryonic inducing factors on heparin-Sepharose.
    Cell Differ. 1987 Jul;21(2):131-6 PMID: 3652212
  11. Purification of heparin-binding growth factors.
    Anal Biochem. 1986 Apr;154(1):1-14 PMID: 3518534
  12. The formation of the mesoderm in urodelean amphibians : V. Its regional induction by the endoderm.
    Wilhelm Roux Arch Entwickl Mech Org. 1973 Dec;173(4):319-332 PMID: 28304800
  13. [Experiments on the chemical identification of embryonal induction substances].
    Hoppe Seylers Z Physiol Chem. 1956 Sep 29;306(1):7-32 PMID: 13376064
  14. Mesoderm-inducing factors. Their possible relationship to heparin-binding growth factors and transforming growth factor-beta.
    Naturwissenschaften. 1987 Dec;74(12):604-6 PMID: 3481645
  15. The inducing capacity of the presumptive endoderm of Xenopus laevis studied by transfilter experiments.
    Roux Arch Dev Biol. 1986 Sep;195(7):467-473 PMID: 28305409
  16. Synergistic induction of mesoderm by FGF and TGF-beta and the identification of an mRNA coding for FGF in the early Xenopus embryo.
    Cell. 1987 Dec 4;51(5):869-77 PMID: 3479265
  17. Formation of mesodermal pattern by secondary inducing interactions.
    Wilehm Roux Arch Dev Biol. 1979 Sep;187(3):231-244 PMID: 28305080
  18. Electrofocusing of the vegetalizing inducing factor.
    Biochim Biophys Acta. 1970 Apr 14;208(1):157-9 PMID: 5441205
  19. Mesoderm induction in early Xenopus embryos by heparin-binding growth factors.
    Nature. 1987 Mar 12-18;326(6109):197-200 PMID: 3821895
  20. An embryonic inducing factor: isolation by high performance liquid chromatography and chemical properties.
    Biol Chem Hoppe Seyler. 1985 Aug;366(8):729-35 PMID: 4063073
  21. A technique for testing macromolecular samples in solution for morphogenetic effects on the isolated ectoderm of the amphibian gastrula.
    Dev Biol. 1961 Aug;3:411-23 PMID: 13787097
  22. [Isolation of ribonucleic acids and nucleotides from embryonal extracts and liver, and their inductor behavior].
    Hoppe Seylers Z Physiol Chem. 1956 Dec 10;306(2-3):132-42 PMID: 13405489
  23. Test of embryonic inducing factors: advantages and disadvantages of different procedures.
    Prog Clin Biol Res. 1986;226:25-34 PMID: 3543958
  24. EXOVAGINATION OF NEWT ENDODERM: CELL AFFINITIES ALTERED BY THE MESODERMAL INDUCING FACTOR.
    Science. 1965 Jan 8;147(3654):167-9 PMID: 14220451
  25. [Action of sulfhydryl compounds on embryonic inducing factors].
    Wilhelm Roux Arch Entwickl Mech Org. 1969 Dec;163(4):316-324 PMID: 28304474
  26. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  27. High performance gel permeation chromatography of proteins. Application to embryonic inducing factors.
    Mol Biol Rep. 1981 Nov 30;8(1):7-10 PMID: 7329417
  28. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  29. The formation of mesodermal derivatives after induction with vegetalizing factor depends on secondary cell interactions.
    Cell Differ. 1980;9(4):229-38 PMID: 7397779
  30. Induction of mesodermal tissues by acidic and basic heparin binding growth factors.
    Cell Differ. 1988 Feb;22(3):183-9 PMID: 2451566
  31. [Experiments on the determination of embryonal amphibian tissue by induction substances in solution].
    Z Naturforsch B. 1959 Aug-Sep;14B:608-9 PMID: 13848979
  32. Purification and properties of a type beta transforming growth factor from bovine kidney.
    Biochemistry. 1983 Dec 6;22(25):5692-8 PMID: 6607069
  33. Establishment of a cell line (XTC-2) from the South African clawed toad, Xenopus laevis.
    Experientia. 1973 Apr 15;29(4):466-7 PMID: 4708349
  34. The simultaneous inducing action of liver and bone-marrow of the guinea-pig in implantation and explantation experiments with embryos of Triturus.
    Exp Cell Res. 1955;(Suppl 3):346-57 PMID: 13344491
Article Info
Journal
Roux's archives of developmental biology : the official organ of the EDBO
Abbr.
Rouxs Arch Dev Biol
ISSN
0930-035X
DOI
10.1007/BF00376364
Published
1989-05-00
Pages
8-13
Language
English
Region
Germany
NLM ID
8606607
External Links
PubMed source DOI: 10.1007/BF00376364
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