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PMID: 28305412 Published · ppublish English Journal Article

Mesodermal induction in early amphibian embryos by activin A (erythroid differentiation factor).

Roux's archives of developmental biology : the official organ of the EDBO ·Vol. 198 ·No. 6 ·1990-03-00 ·Pages 330-335

Asashima M, Nakano H, Shimada K, Kinoshita K, Ishii K, Shibai H, Ueno N

Abstract

Recently the mesoderm-inducing effects of the transforming growth factor β (TGF-β) family of proteins have been widely examined. In an attemt to elucidate the functions of these proteins, porcine inhibin A and activin A (erythroid differentiation factor; EDF) were examined. Treatment of explants with activin A led to differentiation of mesodermal derivatives such as mesenchyme, notochord, blood cells and muscle, but inhibin A had a much lesser effect. The mesodermal differentiation induced by activin A was also comfirmed by analyses using a polyclonal antibody against muscle myosin. By indirect immunofluorescence analysis, the differentiation of muscle blocks was observed in the activin-A-treated explants, whereas no differentiation was observed in inhibin-A-treated and control explants. These findings confirm that this protein of the TGF-β family has mesoderm-inducing ability.

Keywords
Activin A Erythroid differentiation factor Mesoderm induction Xenopus laevis
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Asashima Makoto
Department of Biology, Yokohama City University, 22-2 Seto, Kanazawa-ku, 236, Yokohama, Japan.
Nakano Hiroshi
Department of Biology, Yokohama City University, 22-2 Seto, Kanazawa-ku, 236, Yokohama, Japan.
Shimada Kazunori
Department of Biology, Yokohama City University, 22-2 Seto, Kanazawa-ku, 236, Yokohama, Japan.
Kinoshita Kei
Biological Laboratory, Nippon Medical School, 2-297-2 Kosugi, Nakahara-ku, 211, Kawasaki, Japan.
Ishii Koichi
Central Research Laboratories, Ajinomoto Co. Inc., 1-1 Suzuki, Kawasaki-ku, 210, Kawasaki, Japan.
Shibai Hiroshiro
Central Research Laboratories, Ajinomoto Co. Inc., 1-1 Suzuki, Kawasaki-ku, 210, Kawasaki, Japan.
Ueno Naoto
Institute of Applied Biochemistry, Tsukuba University, Tennodai, Tsukuba, 305, Ibaraki, Japan.
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Article Info
Journal
Roux's archives of developmental biology : the official organ of the EDBO
Abbr.
Rouxs Arch Dev Biol
ISSN
0930-035X
DOI
10.1007/BF00383771
Published
1990-03-00
Pages
330-335
Language
English
Region
Germany
NLM ID
8606607
External Links
PubMed source DOI: 10.1007/BF00383771
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