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PMID: 2829849 Published · ppublish English Journal Article

The dephosphorylation of inositol 1,4-bisphosphate to inositol in liver and brain involves two distinct Li+-sensitive enzymes and proceeds via inositol 4-phosphate.

The Biochemical journal ·Vol. 249 ·No. 1 ·1988-01-01 ·Pages 143-8

Ragan CI, Watling KJ, Gee NS, Aspley S, Jackson RG, Reid GG, Baker R, Billington DC, Barnaby RJ, Leeson PD

Abstract

1. Hydrolysis of both enantiomers of inositol 1-phosphate and both enantiomers of inositol 4-phosphate to inositol is inhibited by LiCl in liver and brain. 2. The phosphatase activity is predominantly soluble. 3. Inositol 1,4-bisphosphate is also hydrolysed by the soluble fraction of liver and brain. 4. Bisphosphatase activity is inhibited by LiCl, but is less sensitive than monophosphatase activity. 5. The product of bisphosphatase in liver and brain is inositol 4-phosphate.

MeSH Terms
Animals Brain/drug effects,enzymology,metabolism Chlorides/pharmacology Chromatography, High Pressure Liquid Hydrolysis In Vitro Techniques Inositol/metabolism Inositol Phosphates/metabolism Lithium/pharmacology Lithium Chloride Liver/drug effects,enzymology,metabolism Phosphoric Monoester Hydrolases/metabolism Rats Sugar Phosphates/metabolism
Chemicals
Chlorides Inositol Phosphates Sugar Phosphates inositol 4-phosphate inositol 1,4-bis(phosphate) Inositol Lithium myo-inositol-4-phosphatase Phosphoric Monoester Hydrolases myo-inositol-1 (or 4)-monophosphatase inositol-1,4-bisphosphate 1-phosphatase Lithium Chloride
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Ragan C I
Department of Biochemistry, Merck Sharp and Dohme Research Laboratories, Harlow, Essex, U.K.
Watling K J
Gee N S
Aspley S
Jackson R G
Reid G G
Baker R
Billington D C
Barnaby R J
Leeson P D
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2490143
Published
1988-01-01
Pages
143-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1148677
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2490143 PMC Full Text View journal details
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