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PMID: 2828228 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In vitro expansion of Epstein-Barr virus-specific HLA-restricted cytotoxic T cells direct from the blood of infectious mononucleosis patients.

Immunology ·Vol. 62 ·No. 4 ·1987-12-00 ·Pages 647-54

Strang G, Rickinson AB

Abstract

The cytotoxic T-cell response induced by primary Epstein-Barr virus (EBV) infection and detectable in the blood of infectious mononucleosis (IM) patients shows several unusual features when tested in in vitro assays. Lysis of EBV-transformed target lines occurs with no apparent HLA restriction, and the putative EBV specificity of the response has been seriously questioned. In the present work we show that the primary T-cell response in IM is polyclonal and indeed does contain a virus-specific HLA class I antigen-restricted component, which can be selectively expanded in vitro in the presence of appropriate stimulator cells and IL-2. This allows functional analysis of the virus-specific component of the response in the absence of co-resident reactivities. Studies on blood samples taken from individuals in the acute phase of IM and again post-convalescence suggest that functionally similar populations of HLA class I-restricted cytotoxic T cells are involved in the control of both the primary and persistent phase of EBV infection.

MeSH Terms
Acute Disease Antigens, Viral/immunology Cell Division Cytotoxicity, Immunologic HLA Antigens/analysis Herpesvirus 4, Human/immunology Humans Immunologic Memory In Vitro Techniques Infectious Mononucleosis/immunology Lymphocyte Activation T-Lymphocytes, Cytotoxic/immunology
Chemicals
Antigens, Viral HLA Antigens
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Strang G
Department of Cancer Studies, University of Birmingham, U.K.
Rickinson A B
References (25)
25 references, click to expand
  1. Epstein-Barr virus: transformation, cytopathic changes, and viral antigens in squirrel monkey and marmoset leukocytes.
    Proc Natl Acad Sci U S A. 1972 Feb;69(2):383-7 PMID: 4333982
  2. Multiple HLA class I-dependent cytotoxicities constitute the "non-HLA-restricted" response in infectious mononucleosis.
    Eur J Immunol. 1987 Jul;17(7):1007-13 PMID: 2440688
  3. Cell-mediated immunity to Epstein-Barr-virus-transformed lymphoblastoid cells in acute infectious mononucleosis.
    N Engl J Med. 1975 Dec 4;293(23):1159-63 PMID: 171568
  4. Studies on cytotoxicity generated in human mixed lymphocyte cultures. I. Time course and target spectrum of several distinct concomitant cytotoxic activities.
    J Immunol. 1978 Apr;120(4):1415-22 PMID: 205612
  5. EBV-related cytotoxicity of Fc receptor negative T lymphocytes separated from the blood of infectious mononucleosis patients.
    Cancer Lett. 1978 Apr;4(4):185-9 PMID: 206347
  6. Fine specificity of a continuously growing killer cell clone specific for H-Y antigen.
    Eur J Immunol. 1979 Aug;9(8):592-7 PMID: 91527
  7. The cellular basis for viral-induced immunodeficiency: analysis by monoclonal antibodies.
    J Immunol. 1980 Sep;125(3):1269-74 PMID: 6251132
  8. Mitotic EBNA-positive lymphocytes in peripheral blood during infectious mononucleosis.
    Nature. 1980 Sep 25;287(5780):334-5 PMID: 6252472
  9. Epstein Barr virus selective T cells in infectious mononucleosis are not restricted to HLA-A and B antigens.
    J Immunol. 1981 Jul;127(1):293-300 PMID: 6263974
  10. Increased numbers of cells with suppressor T cell phenotype in the peripheral blood of patients with infectious mononucleosis.
    Clin Exp Immunol. 1981 Feb;43(2):291-7 PMID: 6456093
  11. Cytotoxic T cell recognition of Epstein-Barr virus-infected B cells. I. Specificity and HLA restriction of effector cells reactivated in vitro.
    Eur J Immunol. 1981 Sep;11(9):686-93 PMID: 6170517
  12. Cytotoxic T cell recognition of Epstein-Barr virus-infected B cells. II. Blocking studies with monoclonal antibodies to HLA determinants.
    Eur J Immunol. 1981 Sep;11(9):694-9 PMID: 6271557
  13. Spontaneous release of a factor with properties of T cell growth factor from a continuous line of primate tumor T cells.
    J Immunol. 1981 Nov;127(5):1852-6 PMID: 6975300
  14. T-cell response to B-cells and Epstein-Barr virus antigens in infectious mononucleosis.
    Cancer Res. 1981 Nov;41(11 Pt 1):4210-5 PMID: 6272964
  15. Stimulation of human lymphocytes with irradiated cells of the autologous Epstein-Barr virus-transformed cell line. I. Virus-specific and nonspecific components of the cytotoxic response.
    Cell Immunol. 1982 Feb;67(1):129-40 PMID: 6176332
  16. Cytotoxic effector cells from infectious mononucleosis patients in the acute phase do not specifically kill Epstein-Barr virus genome-carrying lymphoid cell lines.
    Infect Immun. 1982 Oct;38(1):251-9 PMID: 6292093
  17. Cytotoxic T cell recognition of Epstein-Barr virus-infected B cells. III. Establishment of HLA-restricted cytotoxic T cell lines using interleukin 2.
    Eur J Immunol. 1982 Dec;12(12):1012-8 PMID: 6297919
  18. Epstein-Barr virus-specific cytotoxic T lymphocytes as probes of HLA polymorphism. Heterogeneity of T cell-restricting determinants associated with the serologically defined HLA-A2 antigen.
    J Exp Med. 1983 Aug 1;158(2):280-93 PMID: 6193217
  19. T cell subsets and the recognition of MHC class.
    Immunol Rev. 1983;74:129-42 PMID: 6226585
  20. Cross-reactivity of self-HLA-restricted Epstein-Barr virus-specific cytotoxic T lymphocytes for allo-HLA determinants.
    J Exp Med. 1983 Dec 1;158(6):1804-21 PMID: 6196431
  21. T lymphocytes in infectious mononucleosis. I. T cell death in vitro.
    Clin Exp Immunol. 1985 Apr;60(1):61-9 PMID: 3874017
  22. T lymphocytes in infectious mononucleosis. II. Response in vitro to interleukin-2 and establishment of T cell lines.
    Clin Exp Immunol. 1985 Apr;60(1):70-7 PMID: 3874018
  23. Effects of monoclonal antibodies to the alpha and beta chains of the human lymphocyte function-associated (H-LFA-1) antigen on T lymphocyte functions.
    Eur J Immunol. 1985 Sep;15(9):888-92 PMID: 3930261
  24. Control of human B-lymphocyte replication. I. Characterization of novel activation states that precede the entry of G0 B cells into cycle.
    Immunology. 1986 Aug;58(4):583-9 PMID: 2426188
  25. Cytotoxic effector cells specific for B Cell lines transformed by Epstein-Barr virus are present in patients with infectious mononucleosis.
    Proc Natl Acad Sci U S A. 1975 Apr;72(4):1622-6 PMID: 165518
Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
Published
1987-12-00
Pages
647-54
Language
English
Region
England
NLM ID
0374672
PMCID
PMC1454146
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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