Abstract
The retroviral oncogene v-myb encodes a 45,000 Mr nuclear protein (p45v-myb) that is predominantly associated with the chromatin of transformed cells. It has previously been shown that p45v-myb, when released from chromatin by salt-treatment, binds to DNA. To analyse the biochemical properties of p45v-myb in more detail we have expressed the v-myb coding region in Escherichia coli. Our results demonstrate that bacterially expressed myb protein has an intrinsic DNA-binding activity. Using two alternative strategies, (i) inhibition of DNA-binding by monoclonal antibodies and (ii) analysis of DNA-binding activities of partially deleted forms of the bacterial myb protein, we show that the DNA-binding domain is located in the amino-terminal region of the v-myb protein. This region has been highly conserved between myb genes of different species. Our results are therefore consistent with the hypothesis that DNA-binding is an important aspect of myb protein function.
MeSH Terms
Amino Acid Sequence
Animals
Avian Leukosis Virus/genetics
Avian Myeloblastosis Virus/genetics
Base Sequence
Cell Line
Cloning, Molecular
DNA Restriction Enzymes
DNA-Binding Proteins/genetics,metabolism
Escherichia coli/genetics
Genes
Genes, Viral
Oncogenes
Plasmids
Chemicals
DNA-Binding Proteins
DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Klempnauer K H
Zentrum für Molekulare Biologie, Universität Heidelberg, FRG.
Sippel A E
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