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PMID: 2823263 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and sequence of a cDNA clone for human tyrosinase that maps at the mouse c-albino locus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 21 ·1987-11-00 ·Pages 7473-7

Kwon BS, Haq AK, Pomerantz SH, Halaban R

Abstract

Screening of a lambda gt11 human melanocyte cDNA library with antibodies against hamster tyrosinase (monophenol, L-dopa:oxygen oxidoreductase, EC 1.14.18.1) resulted in the isolation of 16 clones. The cDNA inserts from 13 of the 16 clones cross-hybridized with each other, indicating that they were from related mRNA species. One of the cDNA clones, Pmel34, detected one mRNA species with an approximate length of 2.4 kilobases that was expressed preferentially in normal and malignant melanocytes but not in other cell types. The amino acid sequence deduced from the nucleotide sequence showed that the putative human tyrosinase is composed of 548 amino acids with a molecular weight of 62,610. The deduced protein contains glycosylation sites and histidine-rich sites that could be used for copper binding. Southern blot analysis of DNA derived from newborn mice carrying lethal albino deletion mutations revealed that Pmel34 maps near or at the c-albino locus, the position of the structural gene for tyrosinase.

MeSH Terms
Albinism/genetics Amino Acid Sequence Animals Base Sequence Catechol Oxidase/genetics Cell Line Cloning, Molecular DNA/isolation & purification DNA Restriction Enzymes Genes Humans Melanocytes/enzymology Melanoma/enzymology Mice Molecular Sequence Data Monophenol Monooxygenase/genetics Neuroblastoma/enzymology Nucleic Acid Hybridization
Chemicals
DNA Catechol Oxidase Monophenol Monooxygenase DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kwon B S
Molecular Genetics Laboratory, Guthrie Research Institute, Sayre, PA 18840.
Haq A K
Pomerantz S H
Halaban R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.21.7473
Published
1987-11-00
Pages
7473-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299318
Subset
IM
Grants
NIAID NIH HHS · 1R23AI23058-01 · United States
NCI NIH HHS · CA04679 · United States
NCI NIH HHS · CA07093 · United States
Databases
GENBANK
J03581
Corrections
ErratumIn
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External Links
PubMed source DOI: 10.1073/pnas.84.21.7473 PMC Full Text View journal details
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