Home LiteratureArticle Details
PMID: 3008090 Published · ppublish English Journal Article

Cloning and expression of cDNA encoding mouse tyrosinase.

Nucleic acids research ·Vol. 14 ·No. 6 ·1986-03-25 ·Pages 2413-27

Shibahara S, Tomita Y, Sakakura T, Nager C, Chaudhuri B, Müller R

Abstract

We have isolated a pigment cell-specific cDNA clone from a B16 mouse melanoma cDNA library by differential hybridization. The mRNA of isolated cDNA is highly expressed in B16 melanoma cells and in black mouse (C57BL/6) skin, but is not detectable in mouse neuroblastoma cells nor in K1735 mouse amelanotic melanoma cells. The protein sequence deduced from the nucleotide sequence of the cloned cDNA shows significant similarity to the entire region of Neurospora tyrosinase. To know the identity of cDNA, we transfected K1735 amelanotic melanoma and COS-7 cells with the cDNA carried in a simian virus 40 vector (pKCRH2). We confirmed that the isolated cDNA encodes mouse tyrosinase by immunofluorescence staining of transfected cells using two different anti-T4-tyrosinase monoclonal antibodies. Tyrosinase is composed of 513 amino acids with a molecular weight of 57,872 excluding a hydrophobic signal peptide of 24 amino acids.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Catechol Oxidase/genetics Cloning, Molecular DNA/genetics DNA Restriction Enzymes Gene Expression Regulation Melanins/biosynthesis Melanoma/genetics Mice Monophenol Monooxygenase/genetics Pigmentation
Chemicals
Melanins DNA Catechol Oxidase Monophenol Monooxygenase DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Shibahara S
Tomita Y
Sakakura T
Nager C
Chaudhuri B
Müller R
References (42)
42 references, click to expand
  1. Serological studies on the carbohydrate moiety of human tyrosinase.
    Arch Dermatol Res. 1975 Dec 31;254(3):275-80 PMID: 813583
  2. Transfer of proteins across membranes. II. Reconstitution of functional rough microsomes from heterologous components.
    J Cell Biol. 1975 Dec;67(3):852-62 PMID: 811672
  3. 3' non-coding region sequences in eukaryotic messenger RNA.
    Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
  4. The nature and metabolism of the carbohydrate-peptide linkages of glycoproteins.
    Biochem Soc Symp. 1974;(40):17-26 PMID: 4620382
  5. Neurospora tyrosinase: molecular weight, copper content and spectral properties.
    FEBS Lett. 1976 Oct 15;69(1):157-60 PMID: 136363
  6. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 PMID: 73185
  7. Particulate tyrosinase of human malignant melanoma. Solubilization, purification following trypsin treatment, and characterization.
    Eur J Biochem. 1978 Apr;85(1):137-46 PMID: 416956
  8. Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor.
    Cell. 1978 Jul;14(3):725-31 PMID: 210957
  9. 5,6-Dihydroxyindole is a melanin precursor showing potent cytotoxicity.
    Nature. 1978 Dec 7;276(5688):626-8 PMID: 723948
  10. Speculations on the role of ultraviolet radiation in the development of malignant melanoma.
    J Natl Cancer Inst. 1979 Sep;63(3):541-8 PMID: 112311
  11. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  12. An experimental approach to the chemotherapy of melanoma.
    J Invest Dermatol. 1980 Feb;74(2):63-5 PMID: 7351499
  13. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  14. Processing mechanisms in the biosynthesis of proteins.
    Ann N Y Acad Sci. 1980;343:1-16 PMID: 6994549
  15. New regulators of melanin biosynthesis and the autodestruction of melanoma cells.
    Nature. 1980 Aug 7;286(5773):617-9 PMID: 6772968
  16. Dopachrome conversion: a possible control point in melanin biosynthesis.
    J Invest Dermatol. 1980 Aug;75(2):192-5 PMID: 6774031
  17. Mammalian tyrosinase: isozymic forms of the enzyme.
    Int J Biochem. 1981;13(1):99-103 PMID: 6781947
  18. The structure of eight distinct cloned human leukocyte interferon cDNAs.
    Nature. 1981 Mar 5;290(5801):20-6 PMID: 6163083
  19. SV40-transformed simian cells support the replication of early SV40 mutants.
    Cell. 1981 Jan;23(1):175-82 PMID: 6260373
  20. Internal duplication and evolution of human ceruloplasmin.
    Proc Natl Acad Sci U S A. 1981 May;78(5):2805-9 PMID: 6942404
  21. Demonstration of multiple phenotypic diversity in a murine melanoma of recent origin.
    J Natl Cancer Inst. 1981 Oct;67(4):947-56 PMID: 6944560
  22. Histidine at the active site of Neurospora tyrosinase.
    Biochemistry. 1981 Oct 13;20(21):6029-35 PMID: 6458322
  23. Primary structure of tyrosinase from Neurospora crassa. II. Complete amino acid sequence and chemical structure of a tripeptide containing an unusual thioether.
    J Biol Chem. 1982 Jun 10;257(11):6414-9 PMID: 6210696
  24. High-efficiency cloning of full-length cDNA.
    Mol Cell Biol. 1982 Feb;2(2):161-70 PMID: 6287227
  25. Mammalian tyrosinase catalyzes three reactions in the biosynthesis of melanin.
    Science. 1982 Sep 17;217(4565):1163-5 PMID: 6810464
  26. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  27. Structural requirements of N-glycosylation of proteins. Studies with proline peptides as conformational probes.
    Biochem J. 1983 Feb 1;209(2):331-6 PMID: 6847620
  28. Transfer of tyrosinase to melanosomes in Harding-Passey mouse melanoma.
    Arch Biochem Biophys. 1983 Aug;225(1):75-85 PMID: 6412634
  29. Sequence homology between retroviral reverse transcriptase and putative polymerases of hepatitis B virus and cauliflower mosaic virus.
    Nature. 1983 Oct 27-Nov 2;305(5937):827-9 PMID: 6195530
  30. Expression of functional acetylcholine receptor from cloned cDNAs.
    Nature. 1984 Feb 16-22;307(5952):604-8 PMID: 6320016
  31. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.
    Nucleic Acids Res. 1984 Jan 25;12(2):857-72 PMID: 6694911
  32. Purification of hamster melanoma tyrosinases and structural studies of their asparagine-linked sugar chains.
    Arch Biochem Biophys. 1984 Nov 15;235(1):63-77 PMID: 6437339
  33. Anti-T4-tyrosinase monoclonal antibodies--specific markers for pigmented melanocytes.
    J Invest Dermatol. 1985 Nov;85(5):426-30 PMID: 2414367
  34. Mammalian tyrosinase; the relationship of copper to enzymatic activity.
    J Biol Chem. 1950 Dec;187(2):793-802 PMID: 14803464
  35. The cytotoxicity of melanin precursors.
    Ann N Y Acad Sci. 1963 Feb 15;100:876-86 PMID: 13963743
  36. Subcellular localization of melanin biosynthesis.
    Ann N Y Acad Sci. 1963 Feb 15;100:497-533 PMID: 13992624
  37. Biochemistry of melanin formation.
    Physiol Rev. 1950 Jan;30(1):91-126 PMID: 15403662
  38. The tyrosinases of mouse melanoma. Isolation and molecular properties.
    J Biol Chem. 1971 May 25;246(10):3079-91 PMID: 4995744
  39. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  40. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  41. Tyrosinase as glycoprotein.
    Arch Dermatol Forsch. 1975;252(3):211-6 PMID: 808174
  42. Factors regulating growth and pigmentation of melanoma cells.
    J Invest Dermatol. 1976 Apr;66(4):201-9 PMID: 178804
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.6.2413
Published
1986-03-25
Pages
2413-27
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339673
Subset
IM
Databases
GENBANK
X03687
External Links
PubMed source DOI: 10.1093/nar/14.6.2413 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2