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PMID: 2823066 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Formation of recombinant lacZ+ DNA in conjugational crosses with a recB mutant of Escherichia coli K12 depends on recF, recJ, and recO.

Molecular & general genetics : MGG ·Vol. 209 ·No. 1 ·1987-08-00 ·Pages 135-41

Lloyd RG, Evans NP, Buckman C

Abstract

Conjugational recombination in Escherichia coli was investigated by monitoring synthesis of the lacZ+ product, beta-galactosidase, in crosses between lacZ mutants. We report here that mutation of recB and any combination of recF, recJ, or recO reduces enzyme production by a factor of between 10- and 25-fold whereas mutation of only one of these genes or any combination of recF, recJ, or recO has no more than a 2-fold effect. Mutation of recN has no effect either alone or in combination with the other mutations. We suggest that the products of recF, recJ, and recO may provide an efficient alternative to the RecBCD enzyme for the initiation of recombination in conjugational crosses but that RecBCD activity is needed in this case to produce a viable recombinant product.

MeSH Terms
Bacterial Proteins/genetics Conjugation, Genetic Crosses, Genetic DNA, Recombinant/metabolism Escherichia coli/enzymology,genetics Galactosidases/genetics Genes Genes, Bacterial Mutation beta-Galactosidase/genetics
Chemicals
Bacterial Proteins DNA, Recombinant Galactosidases beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lloyd R G
Department of Genetics, Queens Medical Centre, University of Nottingham, UK.
Evans N P
Buckman C
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34 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00329848
Published
1987-08-00
Pages
135-41
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00329848
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