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PMID: 2822667 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Genetics of type IIa heat-labile enterotoxin of Escherichia coli: operon fusions, nucleotide sequence, and hybridization studies.

Journal of bacteriology ·Vol. 169 ·No. 11 ·1987-11-00 ·Pages 5180-7

Pickett CL, Weinstein DL, Holmes RK

Abstract

Operon fusions for the Escherichia coli heat-labile enterotoxin type IIa (LT-IIa) operon were isolated and characterized. The LT-IIa genes are organized in a transcriptional unit similar to those of cholera toxin (CT) and the closely related E. coli heat-labile toxin type I (LT-I, with subtypes LTh-I and LTp-I). The nucleotide sequence of the LT-IIa genes was determined and compared with the sequences of LTh-I and CT. The A subunit gene of LT-IIa was found to be 57% homologous with the A subunit gene of LTh-I and 55% homologous with the A gene of CT. Most of the homology derived from the region of the A gene which encodes the A1 fragment. The B gene of LT-IIa was not homologous with the B gene of LTh-I or CT. DNA probes containing various portions of the LT-IIa genes and adjacent sequences were used for hybridization studies with restriction endonuclease fragments of DNA from a collection of LT-II-producing strains. These studies showed that a probe containing much of the A subunit gene hybridized well to DNA from the various strains, but a probe for the B subunit gene did not.

MeSH Terms
Amino Acid Sequence Bacterial Toxins/genetics Base Sequence Cloning, Molecular DNA Restriction Enzymes Enterotoxins/genetics Escherichia coli/genetics Escherichia coli Proteins Genes Genes, Bacterial Molecular Sequence Data Nucleic Acid Hybridization Operon
Chemicals
Bacterial Toxins Enterotoxins Escherichia coli Proteins heat-labile enterotoxin, E coli DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pickett C L
Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799.
Weinstein D L
Holmes R K
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.11.5180-5187.1987
Published
1987-11-00
Pages
5180-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213924
Subset
IM
Grants
NIAID NIH HHS · 5R22 AI14107 · United States
Databases
GENBANK
M17894
External Links
PubMed source DOI: 10.1128/jb.169.11.5180-5187.1987 PMC Full Text View journal details
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