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PMID: 2813373 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Glycine tRNA mutants with normal anticodon loop size cause -1 frameshifting.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 20 ·1989-10-00 ·Pages 7979-83

O'Mahony DJ, Mims BH, Thompson S, Murgola EJ, Atkins JF

Abstract

Mutations in the acceptor stem, the 5-methyluridine-pseudouridine-cytidine (TFC) arm, and the anticodon of Salmonella tRNA2Gly can cause -1 frameshifting. The potential for standard base pairing between acceptor stem positions 1 and 72 is disrupted in the mutant sufS627. This disruption may interfere with the interaction of the tRNA with elongation factor-Tu.GTP or an as-yet-unspecified domain of the ribosome. The potential for standard base pairing in part of the TFC stem is disrupted in mutant sufS625. The nearly universal C-61 base of the TFC stem is altered in mutant sufS617, and the TFC loop is extended in mutant sufS605. These changes are expected to interfere with the stability of the TFC loop and its interaction with the D arm. The mutation in mutant sufS605, and possibly other mutants, alters nucleoside modification in the D arm. Three mutants, sufS601, sufS607, and sufS609, have a cytidine substituted for the modified uridine at position 34, the first anticodon position. None of the alterations grossly disrupts in-frame triplet decoding by the mutant tRNAs. The results show that -1 frameshifting in vivo can be caused by tRNAs with normal anticodon loop size and suggest that alternative conformational states of the mutant tRNAs may allow them to read a codon in frame or to shift reading frame.

MeSH Terms
Anticodon/genetics Base Sequence Cloning, Molecular DNA, Bacterial/genetics Molecular Sequence Data Mutation Nucleic Acid Conformation RNA, Transfer/genetics RNA, Transfer, Amino Acid-Specific/genetics RNA, Transfer, Gly/genetics Salmonella/genetics
Chemicals
Anticodon DNA, Bacterial RNA, Transfer, Amino Acid-Specific RNA, Transfer, Gly RNA, Transfer
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
O'Mahony D J
Department of Biochemistry, University College, Cork, Ireland.
Mims B H
Thompson S
Murgola E J
Atkins J F
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.20.7979
Published
1989-10-00
Pages
7979-83
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298196
Subset
IM
Grants
NIGMS NIH HHS · GM21499 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.20.7979 PMC Full Text View journal details
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