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PMID: 279922 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Units of transcription for cytoplasmic RNA in mouse myeloma cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 9 ·1978-09-00 ·Pages 4374-8

Giorno R, Sauerbier W

Abstract

The size of transcription units for several of the abundant cytoplasmic mRNA species in mouse myeloma cells has been analyzed by the ultraviolet light mapping technique. Inactivation kinetics and target size analyses for production of the predominant RNA species indicate that the mRNAs originate in precursor molecules that are 2--14 times larger than the mature mRNA. This estimate of the size of the transcription unit may be a minimum one since it would not take into account promoter-distal sequences if these were not necessary for processing of the mRNA precursor.

MeSH Terms
Cell Line Kinetics Molecular Weight Multiple Myeloma/genetics Neoplasms, Experimental/genetics Nucleic Acid Precursors/genetics Poly A/metabolism RNA, Messenger/genetics RNA, Neoplasm/genetics Transcription, Genetic/radiation effects Ultraviolet Rays
Chemicals
Nucleic Acid Precursors RNA, Messenger RNA, Neoplasm Poly A
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Giorno R
Sauerbier W
References (27)
27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.9.4374
Published
1978-09-00
Pages
4374-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC336117
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.9.4374 PMC Full Text View journal details
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