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PMID: 279921 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Negative control of hemoglobin production in somatic cell hybrids due to heme deficiency.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 9 ·1978-09-00 ·Pages 4354-8

Benoff S, Bruce SA, Skoultchi AI

Abstract

In somatic cell hybrids formed by the fusion of mouse erythroleukemic cells with mouse primary bone marrow cells, retention of the X chromosome contributed by the bone marrow parent is correlated with inhibition of hemoglobin accumulation in response to dimethyl sulfoxide. The inhibition of hemoglobin accumulation is not due to the absence of globin mRNA. Dimethyl sulfoxide-treated hybrid cells accumulate polyribosomal globin mRNA to levels comparable to those of the parental erythroleukemic cells under the same conditions. Heme, or its precursor delta-aminolevulinc acid, can overcome the effects of the bone marrow X chromosome and induce hemoglobin accumulation in the dimethyl sulfoxide-treated hybrid cells. The data suggest that the X chromosome contributed by the bone marrow cells inhibits hemoglobin production by inhibiting inducible heme biosynthesis, most probably at the step catalyzed by delta-aminolevulinic acid synthetase (EC 2.3.1.37).

MeSH Terms
Aminolevulinic Acid/pharmacology Bone Marrow/metabolism Cell Line Dimethyl Sulfoxide/pharmacology Female Genetic Linkage Heme/biosynthesis Hemin/pharmacology Hemoglobins/biosynthesis Hybrid Cells/metabolism Leukemia, Erythroblastic, Acute/metabolism RNA, Messenger/biosynthesis X Chromosome
Chemicals
Hemoglobins RNA, Messenger Heme Hemin Aminolevulinic Acid Dimethyl Sulfoxide
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Benoff S
Bruce S A
Skoultchi A I
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.9.4354
Published
1978-09-00
Pages
4354-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC336113
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.9.4354 PMC Full Text View journal details
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