The effect of erythropoietin on colonial growth of erythroid precursor cells in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 5 ·1974-05-00 ·Pages 1677-80

Cooper MC, Levy J, Cantor LN, Marks PA, Rifkind RA

Abstract

A method is described for the colonial growth, in semi-solid medium, of erythropoietin-responsive erythroid cell precursors. The erythroid cell precursors were isolated by immune hemolysis from fetal mouse liver. Both the number of precursor cells triggered to proliferate and differentiate, and the size of the erythropoietic colonies formed, are directly dependent upon the concentration of erythropoietin included in the culture.

MeSH Terms

Animals Cell Differentiation/drug effects Cell Division/drug effects Cells, Cultured Culture Media Erythropoiesis/drug effects Erythropoietin/pharmacology Fetus Hemoglobins/biosynthesis In Vitro Techniques Liver Mice Mice, Inbred Strains

Chemicals

Culture Media Hemoglobins Erythropoietin

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Cooper M C

Levy J

Cantor L N

Marks P A

Rifkind R A

References (9)

9 references, click to expand

Erythropoietin effects on fetal mouse erythroid cells. I. Cell population and hemoglobin synthesis.
J Cell Biol. 1971 Dec;51(3):585-95 PMID: 5128349
Synthesis of macromolecules during induction of haemoglobin synthesis by erythropoietin.
J Mol Biol. 1969 May 28;42(1):31-41 PMID: 4241283
Globin messenger RNA activity in erythroid precursor cells and the effect of erythropoietin.
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3575-9 PMID: 4509317
Early stimulation of RNA synthesis by erythropoietin in cultures of erythroid precursor cells.
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3189-94 PMID: 4522297
Erythropoiesis in the rat: differential rates of DNA synthesis and cell proliferation.
Science. 1968 Dec 6;162(3858):1132-4 PMID: 5698854
Induction of colonies of hemoglobin-synthesizing cells by erythropoietin in vitro.
Proc Natl Acad Sci U S A. 1971 Jul;68(7):1542-6 PMID: 4104431
Studies on erythropoiesis. XVI. Response to a single dose of erythropoietin in polycythemic mouse.
J Lab Clin Med. 1961 Jan;57:65-72 PMID: 13699502
Purification of erythropoietin-responsive cells by immune hemolysis.
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1337-41 PMID: 4504341
Erythropoietin effects on fetal mouse erythroid cells. II. Nucleic acid synthesis and the erythropoietin-sensitive cell.
J Biol Chem. 1972 Feb 10;247(3):731-5 PMID: 5058223

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.5.1677

Published

1974-05-00

Pages

1677-80

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC388301

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.5.1677 PMC Full Text View journal details

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The effect of erythropoietin on colonial growth of erythroid precursor cells in vitro.

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