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PMID: 278998 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Human (HLA-A and HLA-B) and murine (H-2K and H-2D) histocompatibility antigens are cell surface receptors for Semliki Forest virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 8 ·1978-08-00 ·Pages 3846-50

Helenius A, Morein B, Fries E, Simons K, Robinson P, Schirrmacher V, Terhorst C, Strominger JL

Abstract

The proteins coded for by the HLA-A and HLA-B loci in man and the H-2K and H-2D loci in mice were identified as cell surface receptors for Semliki Forest virus. This conclusion is based on the following observations: (i) Water-soluble octamers of viral coat proteins inhibit the complement-dependent cytotoxicity of antibodies directed against H-2K and H-2D antigens in mouse cells. (ii) Isolated detergent-soluble HLA-A and HLA-B antigens reconstituted in lipid vesicles inhibit the binding of viral proteins to human cells (as do the water-soluble antigens to a lesser extent). (iii) Reconstituted HLA-A and HLA-B vesicles interact in solution with Semliki Forest virus (or with vesicles containing viral spike proteins), as demonstrated by coprecipitation with antisera. (iv) Complexes between viral spoke proteins and HLA-A and HLA-B antigens or H-2K and H-2D antigens can be isolated from the cell surface by utilizing affinity chromatography or immunoprecipitation.

MeSH Terms
Animals Cell Membrane/immunology Cytotoxicity, Immunologic H-2 Antigens HLA Antigens Humans Membrane Proteins/immunology Mice Receptors, Virus Semliki forest virus Viral Proteins/immunology
Chemicals
H-2 Antigens HLA Antigens Membrane Proteins Receptors, Virus Viral Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Helenius A
Morein B
Fries E
Simons K
Robinson P
Schirrmacher V
Terhorst C
Strominger J L
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.8.3846
Published
1978-08-00
Pages
3846-50
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392884
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.8.3846 PMC Full Text View journal details
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