Preferential differentiation of T cell receptor specificities based on the MHC glycoproteins encountered during development. Evidence for positive selection.

The Journal of experimental medicine ·Vol. 169 ·No. 5 ·1989-05-01 ·Pages 1619-30

Marusić-Galesić S, Longo DL, Kruisbeek AM

Abstract

T cells recognize foreign antigens together with those MHC glycoproteins they have encountered during their development in the thymus. How the repertoire of antigen-specific TCRs is selected has not yet been fully defined. We have investigated the T cell repertoire specificities of CD4-CD8+ cytotoxic T cells developing under conditions where one of the class I MHC-encoded molecules is blocked, while other class I-MHC glycoproteins are still expressed. We show that antigen-specific T cells restricted to the blocked class I fail to develop, while generation of other class I-specific T cell proceeds undisturbed. This highly selective perturbation of the T cell receptor repertoire demonstrates that development of CD4-CD8+ T cells with a certain TCR specificity requires expression of particular alleles of class I MHC. Thus, TCR-MHC interactions provide signals essential to the differentiation of precursor T cells.

MeSH Terms

Animals Animals, Newborn/immunology Antibodies, Monoclonal Cell Differentiation Glycoproteins/immunology H-2 Antigens/immunology Immunization, Passive Mice Mice, Inbred BALB C Mice, Inbred C3H Receptors, Antigen, T-Cell/immunology Spleen/cytology,growth & development T-Lymphocytes, Cytotoxic/immunology Thymus Gland/cytology,growth & development Trinitrobenzenes/immunology

Chemicals

Antibodies, Monoclonal Glycoproteins H-2 Antigens H-2K(K) antigen Receptors, Antigen, T-Cell Trinitrobenzenes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Marusić-Galesić S

Division of Cancer Treatment, National Cancer Institute, Bethesda, Maryland 20892.

Longo D L

Kruisbeek A M

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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.169.5.1619

Published

1989-05-01

Pages

1619-30

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2189321

Subset

External Links

PubMed source DOI: 10.1084/jem.169.5.1619 PMC Full Text View journal details

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Preferential differentiation of T cell receptor specificities based on the MHC glycoproteins encountered during development. Evidence for positive selection.

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