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PMID: 2780550 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A CACGTG motif of the Antirrhinum majus chalcone synthase promoter is recognized by an evolutionarily conserved nuclear protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 18 ·1989-09-00 ·Pages 6930-4

Staiger D, Kaulen H, Schell J

Abstract

In the chalcone synthase gene of Antirrhinum majus (snapdragon), 150 base pairs of the 5' flanking region contain cis-acting signals for UV light-induced expression. A nuclear factor, designated CG-1, specifically recognizes a hexameric motif with internal dyad symmetry, CACGTG, located within this light-responsive sequence. Binding of CG-1 is influenced by C-methylation of the CpG dinucleotide in the recognition sequence. CG-1 is a factor found in a variety of dicotyledonous plant species including Nicotiana tabacum, A. majus, Petunia hybrida, Arabidopsis thaliana, and Glycine max. CACGTG motifs contained within trans-acting factor recognition sites in various other plant promoters can interact with CG-1. In addition, the binding site of the human adenovirus major late transcription factor USF can compete for CG-1 binding to the chalcone synthase promoter. This suggests an evolutionary conservation of trans-acting factor recognition sites involved in divergent mechanisms of gene control.

MeSH Terms
Acyltransferases/genetics Base Sequence Biological Evolution Genes Molecular Sequence Data Nuclear Proteins/genetics,metabolism Oligonucleotide Probes Plants/enzymology,genetics Plants, Toxic Promoter Regions, Genetic Protein Binding Tobacco/metabolism
Chemicals
Nuclear Proteins Oligonucleotide Probes Acyltransferases flavanone synthetase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Staiger D
Max-Planck-Institut für Züchtungsforschung, Cologne, Federal Republic of Germany.
Kaulen H
Schell J
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.18.6930
Published
1989-09-00
Pages
6930-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC297964
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.86.18.6930 PMC Full Text View journal details
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