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PMID: 2760208 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Localization of transforming growth factor alpha and its receptor in gastric mucosal cells. Implications for a regulatory role in acid secretion and mucosal renewal.

The Journal of clinical investigation ·Vol. 84 ·No. 3 ·1989-09-00 ·Pages 1017-23

Beauchamp RD, Barnard JA, McCutchen CM, Cherner JA, Coffey RJ

Abstract

Transforming growth factor alpha (TGF alpha) shares with epidermal growth factor (EGF) structural homology (35%), a common cell-surface membrane receptor (TGF alpha/EGF receptor), and a nearly identical spectrum of biological activity, including inhibition of gastric acid secretion. Herein, we report expression of TGF alpha mRNA in normal gastric mucosa of the adult guinea pig, rat, and dog. TGF alpha mRNA was also detected in matched surgically resected gastric mucosa and adjacent gastric carcinoma from 10 patients, and in gastric mucosa adjacent to a benign ulcer from an additional patient. TGF alpha protein was quantitated by radioimmunoassay and was present in tumor and adjacent mucosa. TGF alpha/EGF receptor mRNA was also detected in gastric mucosa from all species studied. Localization of TGF alpha and TGF alpha/EGF receptor mRNA expression was examined in samples of unfractionated guinea pig gastric mucosa and from chief cell-enriched and parietal cell-enriched fractions. All samples exhibited TGF alpha and TGF alpha/EGF receptor expression. The TGF alpha signal was greatest in the parietal cell fraction (5.8-fold increase), but was also enhanced in the chief cell fraction (1.9-fold increase) relative to the unfractionated gastric mucosa. Like TGF alpha expression, TGF alpha/EGF receptor mRNA expression was most intense in the parietal cell-enriched fraction (7.8-fold increase), but was also increased in the chief cell-enriched fraction (2.7-fold increase) relative to the unfractionated guinea pig gastric mucosa. We conclude that TGF alpha and TGF alpha/EGF receptor genes are expressed in normal adult mammalian gastric mucosa. These findings, when interpreted in light of described actions of TGF alpha and EGF, provide evidence that local production of TGF alpha could play an important role in the regulation of acid secretion and mucosal renewal in the stomach.

MeSH Terms
Adult Aged Aged, 80 and over Animals Blotting, Northern Carcinoma/analysis,metabolism DNA Probes Dogs ErbB Receptors/isolation & purification,physiology Gastric Acid/metabolism Gastric Mucosa/metabolism,physiology Guinea Pigs Humans Middle Aged RNA, Messenger/isolation & purification Rats Stomach Neoplasms/analysis,metabolism Transforming Growth Factors/isolation & purification,metabolism,physiology
Chemicals
DNA Probes RNA, Messenger Transforming Growth Factors ErbB Receptors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Beauchamp R D
Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
Barnard J A
McCutchen C M
Cherner J A
Coffey R J
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI114223
Published
1989-09-00
Pages
1017-23
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC329750
Subset
IM
Grants
NCI NIH HHS · CA 46413 · United States
NCI NIH HHS · CA-01309 · United States
External Links
PubMed source DOI: 10.1172/JCI114223 PMC Full Text View journal details
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