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PMID: 2753523 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Pulmonary inflammation and fibrosis in a murine model of asbestosis and silicosis. Possible role of tumor necrosis factor.

Inflammation ·Vol. 13 ·No. 3 ·1989-06-00 ·Pages 329-39

Bissonnette E, Rola-Pleszczynski M

Abstract

Prolonged asbestos and silica inhalation is associated with pulmonary inflammation and fibrosis. Several studies suggest that TNF may play a role in the development of inflammation and fibrosis. We studied TNF production in a murine model of asbestosis and silicosis. Asbestos fibers caused a significant inflammatory response at two weeks and pulmonary fibrosis beginning at one month. Pulmonary inflammation was principally caused by an accumulation of neutrophils (0.88 x 10(5) neutrophils/compared to 0.05 x 10(5) in controls). TNF production by bronchoalveolar cells was higher in asbestos-instilled mice at two weeks, but was significantly diminished in older mice. Pulmonary inflammation was observed until six months in silica-instilled mice. Neutrophils were also the principal protagonists of the inflammation. In this group, severe fibrosis was observed at two weeks. TNF production in silica-instilled mice was similar to controls, possibly due to the presence of large numbers of neutrophils (3.3 x 10(5)/lavage) that could adsorb TNF. In vitro experiments showed an augmentation of TNF production by bronchoalveolar cells in the presence of silica. Taken together, our data suggest that asbestos and silica stimulate alveolar macrophages to produce TNF, which can be involved in pulmonary inflammation and fibrosis.

MeSH Terms
Animals Asbestosis/metabolism,pathology Disease Models, Animal Macrophages/metabolism Mice Mice, Inbred BALB C Neutrophils/metabolism Pulmonary Alveoli/metabolism,pathology Pulmonary Fibrosis/metabolism,pathology Silicosis/metabolism,pathology Tumor Necrosis Factor-alpha/biosynthesis,physiology
Chemicals
Tumor Necrosis Factor-alpha
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bissonnette E
Immunology Division, Faculty of Medicine, University of Sherbrooke, Québec, Canada.
Rola-Pleszczynski M
References (36)
36 references, click to expand
  1. Role of polymorphonuclear leukocytes in silica-induced pulmonary fibrosis.
    Am J Pathol. 1984 Oct;117(1):37-43 PMID: 6486244
  2. Cachectin/tumor necrosis factor stimulates collagenase and prostaglandin E2 production by human synovial cells and dermal fibroblasts.
    J Exp Med. 1985 Dec 1;162(6):2163-8 PMID: 2999289
  3. Pathogenesis of silicosis: current concepts and hypotheses.
    Lung. 1986;164(3):139-54 PMID: 3088337
  4. Regulation of neutrophil migration and superoxide production by recombinant tumor necrosis factors-alpha and -beta: comparison to recombinant interferon-gamma and interleukin-1 alpha.
    Blood. 1987 Oct;70(4):979-84 PMID: 2820532
  5. Bronchoalveolar lavage in pulmonary fibrosis: comparison of cells obtained with lung biopsy and clinical features.
    Thorax. 1980 Jan;35(1):9-18 PMID: 7361291
  6. Production of reactive oxygen metabolites induced by asbestos fibres in human polymorphonuclear leucocytes.
    J Clin Pathol. 1987 Oct;40(10):1189-93 PMID: 3680543
  7. Bronchoalveolar lavage and lung histology. Comparative analysis of inflammatory and immunocompetent cells in patients with sarcoidosis and hypersensitivity pneumonitis.
    Am Rev Respir Dis. 1985 Aug;132(2):400-4 PMID: 3896081
  8. Tumor necrosis factor induction by Sendai virus.
    J Immunol. 1986 Apr 15;136(8):2938-42 PMID: 3007608
  9. Characterization of the bronchoalveolar cellular response in experimental asbestosis. Different reactions depending on the fibrogenic potential.
    Am Rev Respir Dis. 1985 Jan;131(1):144-9 PMID: 3966702
  10. Kinetics of inflammatory and fibrotic pulmonary changes in a murine model of silicosis.
    J Lab Clin Med. 1985 May;105(5):547-53 PMID: 2985721
  11. Stimulation of the adherence of neutrophils to umbilical vein endothelium by human recombinant tumor necrosis factor.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8667-71 PMID: 3866246
  12. Tumor necrosis factor and interleukin-1: role in acute inflammation and microvascular injury.
    J Lab Clin Med. 1987 Dec;110(6):668-81 PMID: 3316455
  13. Tumor necrosis factor (TNF).
    Science. 1985 Nov 8;230(4726):630-2 PMID: 2413547
  14. Biological effects of recombinant human tumor necrosis factor and its novel muteins on tumor and normal cell lines.
    Cancer Res. 1987 Jan 1;47(1):145-9 PMID: 3791201
  15. Assessment of progression of asbestosis in the sheep model by bronchoalveolar lavage and pulmonary function tests.
    Thorax. 1983 Jun;38(6):449-57 PMID: 6879498
  16. Stimulation of neutrophils by tumor necrosis factor.
    J Immunol. 1986 Jun 1;136(11):4220-5 PMID: 3009619
  17. Fibroblast growth enhancing activity of tumor necrosis factor and its relationship to other polypeptide growth factors.
    J Exp Med. 1986 Mar 1;163(3):632-43 PMID: 3512757
  18. Tumor necrosis factor provokes superoxide anion generation from neutrophils.
    Biochem Biophys Res Commun. 1986 Jun 30;137(3):1094-100 PMID: 3015137
  19. Macrophages as a source of tumoricidal activity (tumor-necrotizing factor).
    Infect Immun. 1980 Nov;30(2):523-30 PMID: 7002801
  20. Experimental silicosis: morphologic and biochemical abnormalities produced by intratracheal instillation of quartz into guinea pig lungs.
    Am J Pathol. 1980 Dec;101(3):595-612 PMID: 6255801
  21. Natural killer (NK) cell-derived hematopoietic colony-inhibiting activity and NK cytotoxic factor. Relationship with tumor necrosis factor and synergism with immune interferon.
    J Exp Med. 1985 Nov 1;162(5):1512-30 PMID: 3932579
  22. Tumor necrosis factor (cachectin) is an endogenous pyrogen and induces production of interleukin 1.
    J Exp Med. 1986 Jun 1;163(6):1433-50 PMID: 3486936
  23. Effect of tumour necrosis factor on cultured human melanoma cells.
    Nature. 1975 Dec 25;258(5537):731-2 PMID: 1207755
  24. A microcomputer program for probit analysis of interleukin-2 (IL-2) titration data.
    J Immunol Methods. 1986 Feb 12;86(2):265-77 PMID: 3484770
  25. Fibroblast growth-stimulatory activity released from human monocytes. The contribution of tumour necrosis factor.
    Scand J Immunol. 1987 Dec;26(6):621-9 PMID: 3423739
  26. An endotoxin-induced serum factor that causes necrosis of tumors.
    Proc Natl Acad Sci U S A. 1975 Sep;72(9):3666-70 PMID: 1103152
  27. Oxidants from phagocytes: agents of defense and destruction.
    Blood. 1984 Nov;64(5):959-66 PMID: 6386073
  28. Receptor binding and activation of polymorphonuclear neutrophils by tumor necrosis factor-alpha.
    J Leukoc Biol. 1987 Mar;41(3):196-204 PMID: 3031189
  29. Tumor necrosis factor enhances eosinophil toxicity to Schistosoma mansoni larvae.
    Proc Natl Acad Sci U S A. 1986 Feb;83(4):1055-9 PMID: 3456562
  30. Stimulation of bone resorption and inhibition of bone formation in vitro by human tumour necrosis factors.
    Nature. 1986 Feb 6-12;319(6053):516-8 PMID: 3511389
  31. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro.
    Science. 1985 Nov 22;230(4728):943-5 PMID: 3933111
  32. Properties of interleukin-1 as a complete secretagogue for human neutrophils.
    Biochem Biophys Res Commun. 1985 Aug 15;130(3):1233-40 PMID: 2992505
  33. Tumor necrosis factor-alpha induces increased hydrogen peroxide production and Fc receptor expression, but not increased Ia antigen expression by peritoneal macrophages.
    J Leukoc Biol. 1987 Dec;42(6):704-7 PMID: 3316463
  34. Characterization of human tumor necrosis factor produced by peripheral blood monocytes and its separation from lymphotoxin.
    Int J Cancer. 1985 Jul 15;36(1):69-73 PMID: 3874835
  35. Pulmonary alveolar macrophage function during acute inflammatory lung injury.
    Am Rev Respir Dis. 1987 Jun;135(6):1300-6 PMID: 3035976
  36. Tumor necrosis factor/cachectin interacts with endothelial cell receptors to induce release of interleukin 1.
    J Exp Med. 1986 Jun 1;163(6):1363-75 PMID: 3011946
Article Info
Journal
Inflammation
Abbr.
Inflammation
ISSN
0360-3997
DOI
10.1007/BF00914399
Published
1989-06-00
Pages
329-39
Language
English
Region
United States
NLM ID
7600105
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00914399 View journal details
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