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PMID: 2747647 Published · ppublish English Journal Article

The myristylation signal of p60v-src functionally complements the N-terminal fps-specific region of P130gag-fps.

Molecular and cellular biology ·Vol. 9 ·No. 5 ·1989-05-00 ·Pages 2214-9

Brooks-Wilson AR, Ball E, Pawson T

Abstract

The P130gag-fps protein-tyrosine kinase of Fujinami sarcoma virus contains an N-terminal fps-specific domain (Nfps) that is important for oncogenicity. The N-terminal 14 amino acids of p60v-src, which direct myristylation and membrane association, can replace the gag-Nfps sequences of P130gag-fps (residues 1 to 635), producing a highly transforming src-fps polypeptide. Conversely, gag-Nfps can restore modest transforming activity to a nonmyristylated v-src polypeptide. These results emphasize the modular construction of protein-tyrosine kinases and indicate that Nfps, possibly in conjunction with gag, functions in the subcellular localization of P130gag-fps.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Line, Transformed DNA/genetics Genetic Complementation Test Molecular Sequence Data Myristic Acids/metabolism Oncogene Proteins, Viral/genetics Protein-Tyrosine Kinases/genetics Recombinant Proteins/genetics,metabolism Subcellular Fractions/metabolism
Chemicals
Myristic Acids Oncogene Proteins, Viral Recombinant Proteins DNA Protein-Tyrosine Kinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brooks-Wilson A R
Division of Molecular and Developmental Biology, Mount Sinai Hospital Research Institute, Toronto, Ontario, Canada.
Ball E
Pawson T
References (41)
41 references, click to expand
  1. Normal rat cell lines deficient in nuclear thymidine kinase.
    Virology. 1981 Aug;113(1):408-11 PMID: 7269249
  2. The pathogenicity and defectiveness of PRCII: a new type of avian sarcoma virus.
    Virology. 1981 Jan 15;108(1):1-12 PMID: 6168093
  3. The transforming proteins of Rous sarcoma virus, Harvey sarcoma virus and Abelson virus contain tightly bound lipid.
    Cell. 1982 Dec;31(2 Pt 1):465-74 PMID: 6297767
  4. Cytoplasmic localization of the transforming protein of Fujinami sarcoma virus: salt-sensitive association with subcellular components.
    J Virol. 1983 Feb;45(2):782-91 PMID: 6300435
  5. Isolation of monoclonal antibodies that recognize the transforming proteins of avian sarcoma viruses.
    J Virol. 1983 Nov;48(2):352-60 PMID: 6312092
  6. A fps gene without gag gene sequences transforms cells in culture and induces tumors in chickens.
    J Virol. 1983 Dec;48(3):744-51 PMID: 6605429
  7. Nucleotide sequence of v-fps in the PRCII strain of avian sarcoma virus.
    J Virol. 1984 Apr;50(1):125-31 PMID: 6321783
  8. Monoclonal antibodies to the transforming protein of Fujinami avian sarcoma virus discriminate between different fps-encoded proteins.
    J Virol. 1984 May;50(2):572-8 PMID: 6323756
  9. Identification of functional regions in the transforming protein of Fujinami sarcoma virus by in-phase insertion mutagenesis.
    Cell. 1984 Jun;37(2):549-58 PMID: 6327075
  10. Transforming proteins of fujinami and PRCII avian sarcoma viruses have different subcellular locations.
    Virology. 1984 May;135(1):168-80 PMID: 6328747
  11. Rous sarcoma virus variants that carry the cellular src gene instead of the viral src gene cannot transform chicken embryo fibroblasts.
    Proc Natl Acad Sci U S A. 1984 Jul;81(14):4424-8 PMID: 6087323
  12. Cellular localization of the transforming protein of wild-type and temperature-sensitive Fujinami sarcoma virus.
    J Virol. 1984 Nov;52(2):557-65 PMID: 6092677
  13. Cellular localization of c-fps gene product NCP98.
    J Virol. 1984 Dec;52(3):913-8 PMID: 6092720
  14. A short sequence in the p60src N terminus is required for p60src myristylation and membrane association and for cell transformation.
    Mol Cell Biol. 1984 Sep;4(9):1834-42 PMID: 6092942
  15. Myristic acid, a rare fatty acid, is the lipid attached to the transforming protein of Rous sarcoma virus and its cellular homolog.
    J Virol. 1985 Jan;53(1):7-12 PMID: 2981363
  16. An N-terminal peptide from p60src can direct myristylation and plasma membrane localization when fused to heterologous proteins.
    Nature. 1985 Mar 28-Apr 3;314(6009):374-7 PMID: 3920530
  17. Specific expression of the human cellular fps/fes-encoded protein NCP92 in normal and leukemic myeloid cells.
    Proc Natl Acad Sci U S A. 1985 Apr;82(8):2379-83 PMID: 2986115
  18. Activation of the transformation potential of the cellular fps gene.
    Cell. 1985 Aug;42(1):105-15 PMID: 2990723
  19. Construction and biological analysis of deletion mutants of Fujinami sarcoma virus: 5'-fps sequence has a role in the transforming activity.
    J Virol. 1985 Sep;55(3):660-9 PMID: 2991588
  20. Correspondence between immunological and functional domains in the transforming protein of Fujinami sarcoma virus.
    J Virol. 1985 Sep;55(3):721-7 PMID: 2991592
  21. Mutation of NH2-terminal glycine of p60src prevents both myristoylation and morphological transformation.
    Proc Natl Acad Sci U S A. 1985 Jul;82(14):4625-8 PMID: 2991884
  22. A lysine in the ATP-binding site of P130gag-fps is essential for protein-tyrosine kinase activity.
    EMBO J. 1986 Jan;5(1):69-76 PMID: 3007119
  23. The absence of myristic acid decreases membrane binding of p60src but does not affect tyrosine protein kinase activity.
    J Virol. 1986 May;58(2):468-74 PMID: 3009860
  24. Replacement of insulin receptor tyrosine residues 1162 and 1163 compromises insulin-stimulated kinase activity and uptake of 2-deoxyglucose.
    Cell. 1986 Jun 6;45(5):721-32 PMID: 3518947
  25. Expression of the mammalian c-fes protein in hematopoietic cells and identification of a distinct fes-related protein.
    Mol Cell Biol. 1985 Oct;5(10):2543-51 PMID: 2426571
  26. Activation and suppression of pp60c-src transforming ability by mutation of its primary sites of tyrosine phosphorylation.
    Cell. 1987 Apr 10;49(1):65-73 PMID: 3103925
  27. Tyrosine phosphorylation regulates the biochemical and biological properties of pp60c-src.
    Cell. 1987 Apr 10;49(1):75-82 PMID: 3103926
  28. A conserved domain regulates interactions of the v-fps protein-tyrosine kinase with the host cell.
    Proc Natl Acad Sci U S A. 1987 Dec;84(24):9064-8 PMID: 3480531
  29. The human c-fps/fes gene product expressed ectopically in rat fibroblasts is nontransforming and has restrained protein-tyrosine kinase activity.
    Mol Cell Biol. 1988 Feb;8(2):578-87 PMID: 3352601
  30. The first seven amino acids encoded by the v-src oncogene act as a myristylation signal: lysine 7 is a critical determinant.
    Mol Cell Biol. 1988 Jun;8(6):2435-41 PMID: 2841581
  31. Anatomy of fos proteins.
    Oncogene Res. 1988;3(1):1-7 PMID: 3144693
  32. The six amino-terminal amino acids of p60src are sufficient to cause myristylation of p21v-ras.
    Mol Cell Biol. 1988 Sep;8(9):3960-3 PMID: 3146693
  33. Intracellular distributions of mechanochemical proteins in cultured fibroblasts.
    Proc Natl Acad Sci U S A. 1977 Sep;74(9):3883-7 PMID: 269440
  34. Immunofluorescence on avian sarcoma virus-transformed cells: localization of the src gene product.
    Cell. 1979 Jan;16(1):11-24 PMID: 217542
  35. Localization of the ASV src gene product to the plasma membrane of transformed cells by electron microscopic immunocytochemistry.
    Cell. 1979 Sep;18(1):125-34 PMID: 228858
  36. Evidence that the src gene product of Rous sarcoma virus is membrane associated.
    Virology. 1980 Feb;101(1):25-40 PMID: 6243822
  37. Adhesion plaques of Rous sarcoma virus-transformed cells contain the src gene product.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3514-8 PMID: 6251464
  38. The protein encoded by the transforming gene of avian sarcoma virus (pp60src) and a homologous protein in normal cells (pp60proto-src) are associated with the plasma membrane.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3783-7 PMID: 6253989
  39. Association of pp60src and src protein kinase activity with the plasma membrane of nonpermissive and permissive avian sarcoma virus-infected cells.
    J Virol. 1980 Dec;36(3):805-15 PMID: 6257928
  40. Organization of pp60src and selected cytoskeletal proteins within adhesion plaques and junctions of Rous sarcoma virus-transformed rat cells.
    J Cell Biol. 1981 Jun;89(3):525-35 PMID: 6265469
  41. Immunofluorescent localization of the transforming protein of Rous sarcoma virus with antibodies against a synthetic src peptide.
    Proc Natl Acad Sci U S A. 1982 Sep;79(17):5322-6 PMID: 6291037
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.5.2214-2219.1989
Published
1989-05-00
Pages
2214-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363016
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.9.5.2214-2219.1989 PMC Full Text View journal details
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