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PMID: 273249 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Egg surface glycoprotein receptor for sea urchin sperm bindin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 2 ·1978-02-00 ·Pages 881-5

Glabe CG, Vacquier VD

Abstract

Bindin is an insoluble protein coating the sperm acrosome process and mediating the adhesion of sperm to sea urchin eggs. Milligrams of bindin have been isolated. Here we report the identification, isolation, and partial characterization of a high molecular weight, trypsin-sensitive glycoprotein fraction from the sea urchin egg surface having species-specific affinity for bindin. This glycoprotein may be the egg surface receptor for bindin. The bindin receptor was released from 125-I-labeled eggs by parthenogenetic activation of eggs with ionophore A23187 in the presence of soybean trypsin inhibitor. The receptor has an isoelectric point of 4.02 and a molecular weight in sea water greater than or equal to 5 X 10(6), suggesting that it is an aggregate. It contains 34% neutral sugars, which are galactose and mannose.

MeSH Terms
Adhesiveness Animals Female Glycoproteins/isolation & purification Isoelectric Point Male Membrane Proteins/isolation & purification Molecular Weight Ovum/analysis Receptors, Drug/isolation & purification Sea Urchins Species Specificity Sperm-Ovum Interactions
Chemicals
Glycoproteins Membrane Proteins Receptors, Drug
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Glabe C G
Vacquier V D
References (18)
18 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.2.881
Published
1978-02-00
Pages
881-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411361
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.2.881 PMC Full Text View journal details
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