Protease released from sea urchin eggs at fertilization alters the vitelline layer and aids in preventing polyspermy.

Binding Sites Dithiothreitol/pharmacology Female Fertilization Insemination/drug effects Microscopy, Electron, Scanning Ovum/cytology,drug effects,enzymology Peptide Hydrolases/metabolism,physiology Protease Inhibitors Sea Urchins Soybeans Trypsin/pharmacology Trypsin Inhibitors/pharmacology

Protease Inhibitors Trypsin Inhibitors Peptide Hydrolases Trypsin Dithiothreitol